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Title: Characterization of difference in structure and function of fresh and mastitic bovine milk fat globules
Authors: Verma A.
Ghosh T.
Bhushan B.
Packirisamy G.
Navani, Naveen Kumar
Sarangi, Pranita P.
Ambatipudi, Kiran S.
Published in: PLoS ONE
Abstract: Characterization of milk fat globule (MFG) was performed to investigate the difference in MFG membrane (MFGM) between fresh and mastitis Holstein Friesian cow milk. Lipid distribution investigated by exogenous phospholipids using microscopy showed higher phospholipid content in fresh compared to mastitic MFGM. Xanthine oxidase assay indicative of membrane impairment revealed lower activity in mastitic samples compared to fresh globules. Of note, significantly higher roughness of globule surface and zeta potential was observed in mastitis compared to fresh globules. Influence of globule membrane on the interaction with L. fermentum demonstrated preferential adhesion of bacteria to fresh compared to mastitic globules including enhanced extent of binding. Results of the present study provides an insight of the interfacial changes occurring at the globule surface as well as highlighting the importance of selective bacterial interaction with milk components for the potential development of functional food with relevance to human health. © 2019 Verma et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation: PLoS ONE(2019), 14(8): -
Issue Date: 2019
Publisher: Public Library of Science
ISSN: 19326203
Author Scopus IDs: 57188847653
Author Affiliations: Verma, A., Department of Biotechnology, Indian Institute of Technology, Roorkee, India
Ghosh, T., Department of Biotechnology, Indian Institute of Technology, Roorkee, India
Bhushan, B., Center for Nanotechnology, Indian Institute of Technology, Roork
Funding Details: Funding:ThisworkissupportedbyDepartmentof Biotechnology(DBT),GovernmentofIndia;Grant No.BT/PR12721/AAQ/1/618/2015(KA).AVis supportedbyDBT,GovernmentofIndiafellowship (DBT/2014/IITR-R/110).Thefunderhadnorolein studydesign,datacollectionandanalysis,decision
Corresponding Author: Ambatipudi, K.; Department of Biotechnology, Indian Institute of TechnologyIndia; email:
Appears in Collections:Journal Publications [BT]

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