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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/9610
Title: Molecular etiopathology of naturally occurring reproductive diseases in female goats
Authors: Beena V.
Pawaiya R.V.S.
Gururaj K.
Singh D.D.
Mishra A.K.
Gangwar N.K.
Gupta V.K.
Singh R.
Sharma A.K.
Karikalan M.
Kumar A.
Published in: Veterinary World
Abstract: Aim: The aim of the present study was to investigate the molecular etiopathology of occurrence of reproductive diseases in female goats. Reproductive diseases in goats account for major economic losses to goat farmers in terms of valuable loss of offspring and animal productivity. Materials and Methods: A total of 660 female genitalia were examined for pathological conditions (macroscopic and microscopic lesions). The etiopathological study was carried out for the presence of pathogenic organisms such as Brucella, Chlamydia, and Campylobacter in the uterus and ovary. Based on the microscopic lesions, suspected samples were subjected to diagnostic polymerase chain reaction (PCR) for various etiological agents employing 16srRNA genus specific primers for Campylobacter and Chlamydophila and OMP31 gene-based PCR for Brucella melitensis and nested PCR using ITS-1 gene primers for Toxoplasma gondii. For Brucella suspected samples, immunohistochemistry (IHC) was also performed. Results: In studied female genitalia, 108 (16.30%) showed gross abnormalities with overall 23.32% occurrence of pathological conditions (macroscopic and microscopic lesions). Pathological involvement of the uterus was the highest 68 (62.96%), followed by the ovaries 27 (25%) and other organs. Major uterine condition observed was endometritis (5.60%). In uterine infections, 35 (5.30%) samples were found positive for Campylobacter spp., 12 (1.81%) samples for B. melitensis, and 3 (0.45%) samples were positive for Chlamydophila spp. Among the samples positive for B. melitensis by PCR, 3 were found positive by IHC also. Corynebacterium ovis was detected by PCR using specific primers in a case of hydrosalpinx. It was concluded that many pathological lesions in female genitalia of functional significance play a major role in infertility in goats. Conclusion: The present study concluded that many pathological lesions in female genitalia of functional significance play a major role in infertility in goats. © Beena, et al.
Citation: Veterinary World (2017), 10(8): 964-972
URI: https://doi.org/10.14202/vetworld.2017.964-972
http://repository.iitr.ac.in/handle/123456789/9610
Issue Date: 2017
Publisher: Veterinary World
Keywords: Brucella melitensis
Campylobacter spp.
Chlamydophila spp.
Corynebacterium ovis
Etiopathology
Female genitalia
Goat
Ovary
Reproductive diseases
Uterus
ISSN: 9728988
Author Scopus IDs: 57211553107
6603610897
45060902600
57195764761
57214672235
15765287000
56733968000
57207895915
57203774408
56910194900
57203774508
Author Affiliations: Beena, V., Division of Animal Health, ICAR-Central Institute for Research on Goats, Makhdoom, Farah, Mathura, Uttar Pradesh, 281 122, India
Pawaiya, R.V.S., Division of Animal Health, ICAR-Central Institute for Research on Goats, Makhdoom, Farah, Mathura, Uttar Pradesh, 281 122, India
Gururaj, K., Division of Animal Health, ICAR-Central Institute for Research on Goats, Makhdoom, Farah, Mathura, Uttar Pradesh, 281 122, India
Singh, D.D., Division of Animal Health, ICAR-Central Institute for Research on Goats, Makhdoom, Farah, Mathura, Uttar Pradesh, 281 122, India
Mishra, A.K., Division of Animal Health, ICAR-Central Institute for Research on Goats, Makhdoom, Farah, Mathura, Uttar Pradesh, 281 122, India
Gangwar, N.K., Division of Animal Health, ICAR-Central Institute for Research on Goats, Makhdoom, Farah, Mathura, Uttar Pradesh, 281 122, India
Gupta, V.K., Centre for Animal Disease Research and Diagnosis (CADRAD), ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, 243 122, India
Singh, R., Division of Pathology, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, 243 122, India
Sharma, A.K., Division of Pathology, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, 243 122, India
Karikalan, M., Division of Pathology, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, 243 122, India
Kumar, A., Division of Animal Health, ICAR-Central Institute for Research on Goats, Makhdoom, Farah, Mathura, Uttar Pradesh, 281 122, India
Corresponding Author: Pawaiya, R.V.S.; Division of Animal Health, ICAR-Central Institute for Research on GoatsIndia; email: rvspawaiya@gmail.com
Appears in Collections:Journal Publications [MS]

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