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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/9497
Title: Expression and determination of immunization dose of recombinant tropomyosin protein of Hyalomma anatolicum for the development of anti-tick vaccine
Authors: Manjunathachar H.V.
Saravanan B.C.
Kumar B.
Tamilmahan P.
Sharma A.K.
Shinde S.
Nandi A.
Nagar G.
Chigure G.
Mohmad A.
Fular A.
Kumar S.
Ghosh S.
Published in: Indian Journal of Animal Sciences
Abstract: Present investigation was carried out to standardize the immunization dose of one recombinant antigen in rat model before conducting large animal experimentation. Tropomyosin (TPM), a muscle associated and highly conserved protein found in all species of invertebrates, was cloned, expressed in prokaryotic system and the downstream process was standardized. SDS-PAGE analysis showed a distinct band of approximately 51 kDa. Western blot analysis using specific sera gave a strong reaction of approximately the same size as that of SDSPAGE. For standardization of immunization dose, the rTPM at three different dosages viz., 150, 300, 450 μg was used to immunize wister rats and the antibody response was titrated by ELISA. Applying ANOVA, highly significant difference in anti-rTPM titre was recorded between the animals injected with 300 μg total dose (TD) and other dosages selected for the study. The significantly high antibody tire at 1:25600 dilution observed in animals immunized with 300 μg TD was selected for further study on in vivo immunization of calves and experimental challenge by the tick stages.
Citation: Indian Journal of Animal Sciences (2017), 87(3): 275-279
URI: http://repository.iitr.ac.in/handle/123456789/9497
Issue Date: 2017
Publisher: Indian Council of Agricultural Research
Keywords: Degenerate primers Hyalomma anatolicum
PRham expression vector
Tropomyosin
Vaccine target
ISSN: 3678318
Author Scopus IDs: 55598123600
15047118600
7401973057
55661369300
57203774408
44462076100
55908698200
36104481700
35108698600
57193825752
57193544572
55811766500
55574183199
Author Affiliations: Manjunathachar, H.V., ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, 243 122, India
Saravanan, B.C., ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, 243 122, India
Kumar, B., ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, 243 122, India
Tamilmahan, P., ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, 243 122, India
Sharma, A.K., ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, 243 122, India
Shinde, S., ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, 243 122, India
Nandi, A., ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, 243 122, India
Nagar, G., ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, 243 122, India
Chigure, G., ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, 243 122, India
Mohmad, A., ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, 243 122, India
Fular, A., ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, 243 122, India
Kumar, S., ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, 243 122, India
Ghosh, S., ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, 243 122, India
Appears in Collections:Journal Publications [MS]

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