Skip navigation
Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/9368
Title: Apoptosis and lipid peroxidation in ochratoxin A- and citrinin-induced nephrotoxicity in rabbits
Authors: Kumar M.
Dwivedi P.
Sharma A.K.
Sankar M.
Patil R.D.
Singh N.D.
Published in: Toxicology and Industrial Health
Abstract: Ochratoxin A (OTA) and citrinin (CIT) are nephrotoxic mycotoxins produced mainly by fungal species Aspergillus ochraceus and Penicillium citrinum, respectively, which have been found to occur together in various food and feed commodities. In the present study, both OTA and CIT were evaluated for their potential to induce oxidative damage by determining lipid peroxidation (LPO) through malondialdehyde (MDA) assay and apoptosis by flow cytometry, gel electrophoresis and renal ultrastructural morphology in rabbits fed with diets containing OTA (0.75 mg/kg feed), CIT (15 mg/kg feed) and OTA + CIT (0.75 and 15 mg/kg feed, respectively) up to 60 days. The concentration of MDA was found significantly higher in OTA and combination-treated groups. OTA and combination-treated groups revealed more apoptotic cells in flow cytometry when compared with the CIT-treated group. Characteristic DNA fragmentation, as evidenced by ladder pattern in electrophoresis appeared in the toxin-treated groups. Ultrastructurally, interstitial cells showed nuclear fragmentation and cytoplasmic blebbing in OTA- and CIT-treated groups; whereas, proximal convoluted tubular epithelial cells, besides interstitial cells, showed nuclear fragmentation in the combined treatment group. The results suggested that low concentrations of OTA and CIT either alone or in combination induced apoptosis in a time-dependent manner and LPO in the rabbit kidney, which appeared to play a major role in the pathogenesis of nephrotoxicity. Furthermore, the interaction of these two nephrotoxic mycotoxins was found to be additive. © 2012, SAGE Publications. All rights reserved.
Citation: Toxicology and Industrial Health (2014), 30(1): 90-98
URI: https://doi.org/10.1177/0748233712452598
http://repository.iitr.ac.in/handle/123456789/9368
Issue Date: 2014
Keywords: Apoptosis
citrinin
lipid peroxidation
nephrotoxicity
ochratoxin A
ISSN: 7482337
Author Scopus IDs: 55617246300
57210229309
57203774408
18038489400
26429964700
57193824761
Author Affiliations: Kumar, M., Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, India
Dwivedi, P., Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, India
Sharma, A.K., Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, India
Sankar, M., Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, India
Patil, R.D., Department of Pathology, College of Veterinary Sciences, Palampur, India
Singh, N.D., Department of Pathology, College of Veterinary Sciences, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India
Funding Details: This study was supported in part by the NATP-CGP (ICAR) Project No. II/221.
Corresponding Author: Kumar, M.; High Security Animal Disease Laboratory, Indian Veterinary Research Institute, Anand Nagar, Bhopal 462021, India
Appears in Collections:Journal Publications [MS]

Files in This Item:
There are no files associated with this item.
Show full item record


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.