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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/9366
Title: Anti-inflammatory effect of alcoholic extract of Entada pursaetha DC in LPS-induced inflammation in mice and RAW264.7 cells
Authors: Kumari R.R.
Lingaraju M.C.
Gupta G.
More A.S.
Balaganur V.
Kumar D.
Kumar P.
Kumar D.
Sharma A.K.
Mishra S.K.
Tandan S.K.
Published in: Indian Journal of Biochemistry and Biophysics
Abstract: Entada pursaetha (Fabaceae) is an active, oriental herbal medicine used to treat various inflammatory diseases. Limited information on the use of stem in ethno medicine in painful conditions of joints prompted us to verify its usage for in vitro and in vivo anti-inflammatory action. The present study was designed to evaluate the anti-inflammatory effects of ethanolic extract of E. pursaetha (EPE) on the production of inflammatory mediators in macrophages and LPS (lipopolysaccharide)- induced systemic inflammation. EPE was studied for its anti-inflammatory activity in on the production of inflammatory mediators in systemic inflammation triggered by LPS and LPS-stimulated RAW 264.7 cells. Plasma tumor necrosis factor alpha (TNF-α), interleukin 1 beta (IL-1β) proteins and total nitrate and nitrite levels were significantly increased in vehicletreated control group of mice challenged with LPS (4 mg/kg b.wt.). Pre treatment of EPE at 300 mg/kg b.wt. Continuously for 3-days prior to LPS challenge significantly reduced these cytokine levels and nitrate plus nitrite levels in plasma. EPE treatment also significantly inhibited the LPS-induced production of NO (nitric oxide), IL-1β and TNF-α in RAW 264.7 cell. This study demonstrates the anti-inflammatory effects of EPE in both in vivo and in vitro model of inflammation potentially justifying the usage of Entada pursaetha plant as traditional herbal medicine for the treatment of inflammatory diseases. © 2017, National Institute of Science Communication and Information Resources (NISCAIR) . All rights reserved.
Citation: Indian Journal of Biochemistry and Biophysics (2017), 54(43924): 140-149
URI: http://repository.iitr.ac.in/handle/123456789/9366
Issue Date: 2017
Publisher: National Institute of Science Communication and Information Resources (NISCAIR)
Keywords: Anti-inflammatory activity
Cytokines
Entada pursaetha
Lipopolysaccharide
RAW 264.7 cells
ISSN: 3011208
Author Scopus IDs: 35074470800
55581017800
15047988400
56543022400
55581181300
57211016614
57214054903
57211196210
57203774408
14319398500
55665027400
Author Affiliations: Kumari, R.R., Division of Pharmacology and Toxicology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh 243 122, India
Lingaraju, M.C., Division of Pharmacology and Toxicology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh 243 122, India
Gupta, G., Division of Pharmacology and Toxicology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh 243 122, India
More, A.S., Division of Pharmacology and Toxicology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh 243 122, India
Balaganur, V., Division of Pharmacology and Toxicology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh 243 122, India
Kumar, D., Division of Pharmacology and Toxicology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh 243 122, India
Kumar, P., Division of Medicine, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh 243 122, India
Kumar, D., Division of Pharmacology and Toxicology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh 243 122, India
Sharma, A.K., Division of Pathology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh 243 122, India
Mishra, S.K., Division of Pharmacology and Toxicology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh 243 122, India
Tandan, S.K., Division of Pharmacology and Toxicology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh 243 122, India
Corresponding Author: Tandan, S.K.; Division of Pharmacology and Toxicology, Indian Veterinary Research Institute, Izatnagar, India; email: sktandan@gmail.com
Appears in Collections:Journal Publications [MS]

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