Skip navigation
Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/7331
Title: Enhanced hypoxic tolerance by Seabuckthorn is due to upregulation of HIF-1? and attenuation of er stress
Authors: Jain K.
Suryakumar G.
Prasad R.
Ganju L.
Singh S.B.
Published in: Journal of Applied Biomedicine
Abstract: An imbalance in the redox homeostasis causes activation of multifaceted signaling responses which may be protective or deleterious. Amelioration of oxidative stress is one of the major modes of action of herbal supplements like Seabuckthorn (SBT). While the antioxidant potential of SBT is known, investigations into its effect on stress inducible signaling cascades are in progress. Here, we examine the impact of SBTonhypoxic tolerance and the mechanism behind its cardioprotective action. The efficacy of SBT was evaluated using the onset of gasping time (GT) at an altitude of 9754 m as the indicator for hypoxic tolerance. SBT led to a 100% increase in GT and curtailed hypoxia induced cardiac damage and free radical production. SBT upregulated HIF-1? and ledtoatwo-fold increasein HO-1. A 100% increase in NO levels was observed. SBT reduced protein carbonylation and enhanced HSP70 levels. A statistically significant decline was seen in the markers of ER stress, GRP78, PERK and CHOP. SBT potentiated anti-inflammatory effects and downregulated NF-?B and TNF-a. Our study provides a novel insight into the mechanism behind the pro-survival effects of SBT against hypoxia, highlighting the cross talk between key adaptive responses mediated by HIF-1? and ER stress. © 2015 Faculty of Health and Social Studies, University of South Bohemia in Ceske Budejovice. Published by Elsevier Sp. z o.o. All rights reserved.
Citation: Journal of Applied Biomedicine (2016), 14(1): 71-83
URI: https://doi.org/10.1016/j.jab.2015.10.001
http://repository.iitr.ac.in/handle/123456789/7331
Issue Date: 2016
Publisher: University of South Bohemia
Keywords: Caspase
Hif-1?
Hypoxia
Nitric oxide
Oxidative stress
ISSN: 1214021X
Author Scopus IDs: 55638631100
25624005000
57212702134
6603372091
36007701500
Author Affiliations: Jain, K., Cellular Biochemistry Division, Defence Institute of Physiology and Allied Sciences, Lucknow Road, Timarpur, Delhi, 110 054, India
Suryakumar, G., Cellular Biochemistry Division, Defence Institute of Physiology and Allied Sciences, Lucknow Road, Timarpur, Delhi, 110 054, India
Prasad, R., Cellular Biochemistry Division, Defence Institute of Physiology and Allied Sciences, Lucknow Road, Timarpur, Delhi, 110 054, India
Ganju, L., Cellular Biochemistry Division, Defence Institute of Physiology and Allied Sciences, Lucknow Road, Timarpur, Delhi, 110 054, India
Singh, S.B., Cellular Biochemistry Division, Defence Institute of Physiology and Allied Sciences, Lucknow Road, Timarpur, Delhi, 110 054, India
Funding Details: The study was supported by Defence Research and Development Organisation, Ministry of Defence, Government of India . The first author is a Council of Scientific and Industrial Research Senior Research Fellow.
Corresponding Author: Suryakumar, G.; Cellular Biochemistry Division, Defence Institute of Physiology and Allied Sciences, Lucknow Road, India; email: geethasuryakumar@yahoo.com
Appears in Collections:Journal Publications [EE]

Files in This Item:
There are no files associated with this item.
Show full item record


Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.