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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/6338
Title: Ancient DNA reveals Late Pleistocene existence of ostriches in Indian sub-continent
Authors: Jain S.
Rai N.
Kumar G.
Pruthi P.A.
Thangaraj K.
Bajpai S.
Pruthi V.
Published in: PLoS ONE
Abstract: Ancient DNA (aDNA) analysis of extinct ratite species is of considerable interest as it provides important insights into their origin, evolution, paleogeographical distribution and vicariant speciation in congruence with continental drift theory. In this study, DNA hotspots were detected in fossilized eggshell fragments of ratites (dated ?25000 years B.P. by radiocarbon dating) using confocal laser scanning microscopy (CLSM). DNA was isolated from five eggshell fragments and a 43 base pair (bp) sequence of a 16S rRNA mitochondrial-conserved region was successfully amplified and sequenced from one of the samples. Phylogenetic analysis of the DNA sequence revealed a 92% identity of the fossil eggshells to Struthio camelus and their position basal to other palaeognaths, consistent with the vicariant speciation model. Our study provides the first molecular evidence for the presence of ostriches in India, complementing the continental drift theory of biogeographical movement of ostriches in India, and opening up a new window into the evolutionary history of ratites. © 2017 Jain et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation: PLoS ONE (2017), 12(3): -
URI: https://doi.org/10.1371/journal.pone.0164823
http://repository.iitr.ac.in/handle/123456789/6338
Issue Date: 2017
Publisher: Public Library of Science
ISSN: 19326203
Author Scopus IDs: 57209870089
55777761600
55430377500
25958285300
6603011487
16214305000
18837702800
Author Affiliations: Jain, S., Department of Biotechnology, Indian Institute of Technology, Roorkee, Uttarakhand, India
Rai, N., CSIR-Centre for Cellular and Molecular Biology, Hyderabad, India
Kumar, G., Dayalbagh Educational Institute, Dayalbagh, Agra, UP, India
Pruthi, P.A., Department of Biotechnology, Indian Institute of Technology, Roorkee, Uttarakhand, India
Thangaraj, K., CSIR-Centre for Cellular and Molecular Biology, Hyderabad, India
Bajpai, S., Department of Earth Sciences, Indian Institute of Technology, Roorkee, Uttarakhand, India, Birbal Sahni Institute of Palaeosciences, Lucknow, UP, India
Pruthi, V., Department of Biotechnology, Indian Institute of Technology, Roorkee, Uttarakhand, India
Funding Details: For the financial support, we would like to acknowledge the Council for Scientific and Industrial Research (CSIR) and University Grants Commission (UGC), New Delhi.
Appears in Collections:Journal Publications [ES]

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