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Title: Spatial distribution of major bacterial species and different volatile fatty acids in a two-phase anaerobic biofilm reactor with PVA gel beads as bio-carrier
Authors: Pandey S.
Sarkar, Sudipta
Published in: Preparative Biochemistry and Biotechnology
Abstract: Conventional completely mixed anaerobic treatment systems limit the chances of the different species of bacteria to spatially group together according to their mutual cooperation and as a result, show a lower efficiency and vulnerability towards shock situations. It is interesting to know about the stratification of the different bacterial species participating in the degradation process and the intermediates that they produce. In this study, we established and optimized a two-phase anaerobic packed bed biofilm reactor system (AnPBR) with porous PVA gel beads used as bio-carriers and ran the reactor system in a steady state to observe the VFAs produced along with the microbial diversity of the predominant species at different stages of the reactor system. We observed that acetate and butyrate were the predominant intermediate VFAs while concentrations of other VFAs such that propionic acid were low. Acetobacterium and Clostridium were found to be the most abundant bacterial species in acidogenic reactor while methanogenic reactor was highly enriched with Methanobacterium and Methanosarcina. Apart from the above, syntrophic populations such as Syntrophobactor wolinii were also observed to be dominant in both the reactors–especially towards the end of acidogenic reactor and the initial part of the methanogenic reactor. © 2019, © 2019 Taylor & Francis Group, LLC.
Citation: Preparative Biochemistry and Biotechnology(2019), 49(7): 704-717
Issue Date: 2019
Publisher: Taylor and Francis Inc.
Keywords: 16s rDNA
anaerobic biofilm reactor
anaerobic wastewater treatment
microbial diversity
PVA gel beads
two-phase anaerobic reactor
ISSN: 10826068
Author Scopus IDs: 57149849600
Author Affiliations: Pandey, S., Department of Civil Engineering, VSB Engineering College, Karur, India
Sarkar, S., Department of Civil Engineering, Indian Institute of Technology, Roorkee, India
Funding Details: The authors are thankful to Mr. Vipin rose, Head of operations, Kuraray India Private Ltd, for providing us with the PVA-gel beads. Authors are also indebted to Aldee water Pvt. Ltd., Ahmedabad, for their valuable help during the work. One of the authors,
Corresponding Author: Pandey, S.; Department of Civil Engineering, VSB Engineering CollegeIndia; email:
Appears in Collections:Journal Publications [CE]

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