http://repository.iitr.ac.in/handle/123456789/5085| Title: | Purification and characterization of 2s albumin from seeds of Wrightia tinctoria exhibiting antibacterial and DNase activity |
| Authors: | Sharma A. Kumar, P. Kesari P. Neetu Katiki M. Mishra M. Singh P.K. Gurjar, Bhola Ram Sharma, Ashwani Kumar Tomar, Shailly Kumar, Pravindra R.Manish |
| Published in: | Protein and Peptide Letters |
| Abstract: | 2S albumin is a low-molecular-weight seed storage protein belonging to the prolamin superfamily. In the present work a small 2S albumin (WTA) protein of ?16 kDa has been purified from the seeds of Wrightia tinctoria. The WTA is a heterodimer protein with a small subunit of ?5 kDa and a larger subunit of ?11 kDa bridged together through disulphide bonds. The protein exhibits deoxyribonucleases activity against closed circular pBR322 plasmid DNA and linear BL21 genomic DNA. The protein also showed antibacterial activity against Morexalla catarrhalis. CD studies indicate a high ?-helical content in the protein. The conserved disulphide bonds in the protein suggest that the WTA is highly stable under high pH and temperature like other 2S albumin. © 2017 Bentham Science Publishers. |
| Citation: | Protein and Peptide Letters(2017), 24(4): 368-378 |
| URI: | https://doi.org/10.2174/0929866524666170126144936 http://repository.iitr.ac.in/handle/123456789/5085 |
| Issue Date: | 2017 |
| Publisher: | Bentham Science Publishers B.V. |
| Keywords: | 2S albumin B-cell epitope Deoxyribonucleases activity IgE binding allergen Seed storage protein Wrightia tinctoria |
| ISSN: | 9298665 |
| Author Scopus IDs: | 54795869500 57204102455 56604951200 57193241611 57112522900 56357223700 55252883200 8665885900 57214355701 57203506001 55064809000 |
| Author Affiliations: | Sharma, A., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667, India Kumar, P., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667, India Kesari, P., Depart |
| Funding Details: | This work was supported by grant from Department of Biotechnology, Ministry of science and Technology, Government of India (Project No. BT/PR3989/BRB/10/990/2011). PK and Neetu would like to thank CSIR; AS, PK and MK would like to thank MHRD. CD studies a |
| Corresponding Author: | Kumar, P.; Department of Biotechnology, Indian Institute of Technology RoorkeeIndia; email: pravshai@gmail.com |
| Appears in Collections: | Journal Publications [BT] Journal Publications [CE] |
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