Solid, solution, and antitumor activity studies of mixed-ligand complexes with adenine-5-bromouracil base pair

Abstract

Mixed-ligand complexes of Co(II), Ni(Il), Cu(II), Zn(II) and Cd(II) with adenine as primary ligand and 5-bromouracil as secondary ligand were prepared in aqueous ethanol solution at pH of about 7 and subjected to a screening system of Dalton's Lymphoma (DL) tumor cells in vitro for the evaluation of viability, 3 H-thymidine incorporation and induction of apoptosis in tumor cells. The isolated complexes were characterized by various physico-chemical methods. Solution studies were performed pH-metrically for the interaction of the above metal ions with adenine and 5-bromouracil separately (binary) and in the presence of each other (ternary) at 25 ± 0.1°C temperature and a constant ionic strength of 0.1 M NaNO 3 in aqueous solution.