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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/3813
Title: A novel nanohybrid for cancer theranostics: Folate sensitized Fe2O3 nanoparticles for colorectal cancer diagnosis and photodynamic therapy
Authors: Nandi R.
Mishra S.
Maji T.K.
Manna K.
Kar P.
Banerjee S.
Dutta S.
Sharma S.K.
Lemmens P.
Saha K.D.
Pal S.K.
Published in: Journal of Materials Chemistry B
Abstract: Organic-inorganic nanohybrids are becoming popular for their potential biological applications, including diagnosis and treatment of cancerous cells. The motive of this study is to synthesise a nanohybrid for the diagnosis and therapy of colorectal cancer. Here we have developed a facile and cost-effective synthesis of folic acid (FA) templated Fe2O3 nanoparticles with excellent colloidal stability in water using a hydrothermal method for the theranostics applications. The attachment of FA to Fe2O3 was confirmed using various spectroscopic techniques including FTIR and picosecond resolved fluorescence studies. The nanohybrid (FA-Fe2O3) is a combination of two nontoxic ingredients FA and Fe2O3, showing remarkable photodynamic therapeutic (PDT) activity in human colorectal carcinoma cell lines (HCT 116) via generation of intracellular ROS. The light induced enhanced ROS activity of the nanohybrid causes significant nuclear DNA damage, as confirmed from the comet assay. Assessment of p53, Bax, Bcl2, cytochrome c (cyt c) protein expression and caspase 9/3 activity provides vivid evidence for cell death via an apoptotic pathway. In vitro magnetic resonance imaging (MRI) experiments in folate receptor (FR) overexpressed cancer cells (HCT 116) and FR deficient human embryonic kidney cells (HEK 293) reveal the target specificity of the nanohybrid towards cancer cells, and are thus pronounced MRI contrasting agents for the diagnosis of colorectal cancer. © 2017 The Royal Society of Chemistry.
Citation: Journal of Materials Chemistry B (2017), 5(21): 3927-3939
URI: https://doi.org/10.1039/c6tb03292c
http://repository.iitr.ac.in/handle/123456789/3813
Issue Date: 2017
Publisher: Royal Society of Chemistry
ISSN: 20507518
Author Scopus IDs: 57194700396
57190809231
57206413100
54893542000
22834801000
57209162550
56037524500
56374137900
7006256349
14820040000
35351272500
Author Affiliations: Nandi, R., Department of Chemical, Biological and Macromolecular Sciences, S. N. Bose National Centre for Basic Sciences, Salt Lake, Kolkata, 700106, India
Mishra, S., Cancer and Cell Biology Division, CSIR Indian Institute of Chemical Biology, Raja S.C Mullick Road, Kolkata, 700032, India
Maji, T.K., Department of Chemical, Biological and Macromolecular Sciences, S. N. Bose National Centre for Basic Sciences, Salt Lake, Kolkata, 700106, India
Manna, K., Cancer and Cell Biology Division, CSIR Indian Institute of Chemical Biology, Raja S.C Mullick Road, Kolkata, 700032, India
Kar, P., Department of Chemical, Biological and Macromolecular Sciences, S. N. Bose National Centre for Basic Sciences, Salt Lake, Kolkata, 700106, India
Banerjee, S., Cancer and Cell Biology Division, CSIR Indian Institute of Chemical Biology, Raja S.C Mullick Road, Kolkata, 700032, India
Dutta, S., Department of Chemical, Biological and Macromolecular Sciences, S. N. Bose National Centre for Basic Sciences, Salt Lake, Kolkata, 700106, India
Sharma, S.K., Eko X-Ray and Imaging Institute, 54, Jawaharlal Nehru Road, Kolkata, 700071, India
Lemmens, P., Institute for Condensed Matter Physics, Laboratory for Emerging Nanometrology, TU Braunschweig, Mendelssohnstrasse 3, Braunschweig, 38106, Germany
Saha, K.D., Cancer and Cell Biology Division, CSIR Indian Institute of Chemical Biology, Raja S.C Mullick Road, Kolkata, 700032, India
Pal, S.K., Department of Chemical, Biological and Macromolecular Sciences, S. N. Bose National Centre for Basic Sciences, Salt Lake, Kolkata, 700106, India
Funding Details: P. Kar thanks the Council of Scientific and Industrial Research (CSIR, India) for fellowship. T. K. Maji thanks of DST, INSPIRE for fellowship. We thank DAE (India) for financial grant 2013/37P/73/BRNS. We also thank DBT (India) for the financial grant (BT/PR11534/NNT/28/766/2014).
Corresponding Author: Saha, K.D.; Cancer and Cell Biology Division, CSIR Indian Institute of Chemical Biology, Raja S.C Mullick Road, India; email: krishna@iicb.res.in
Appears in Collections:Journal Publications [CY]

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