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Title: Development of membrane electrodes for selective determination of some antiepileptic drugs in pharmaceuticals, plasma and urine
Authors: Gupta V.K.
Singh A.K.
Gupta B.
Published in: Analytical and Bioanalytical Chemistry
Abstract: Newly developed, simple, low-cost and sensitive ion-selective electrodes have been proposed for determination of some antiepileptic drugs such as lamotrigine, felbamate, and primidone in their pharmaceutical preparations as well as in biological fluids. The electrodes are based on poly(vinyl chloride) membranes doped with drug-tetraphenyl borate (TPB) or drug-phosphotungstic acid (PT) ion-pair complexes as molecular recognition materials. The novel electrodes displayed rapid Nernstian responses with detection limits of approximately 10-7 M. Calibration graphs were linear over the ranges 5.2∈×∈10-7-1.0∈×∈10-3, 1.5∈×∈10-6-1.0∈×∈10-3, and 2.6∈×∈10-7-1.0∈×∈10-3M for drug-TPB and 5.8∈×∈10-7-1. 0∈×∈10-3, 1.8∈×∈10 -7-1.0∈×∈10-3, and 6. 6∈×∈10-7-1.0∈×∈10-3 M for drug-PT electrodes, respectively, with slopes ranging from 52.3 to 62.3 mV/decade. The membranes developed have potential stability for up to 1 month and proved to be highly selective for the drugs investigated over other ions and excipients. The results show that the selectivity of the ion-selective electrodes is influenced significantly by the plasticizer. The proposed electrodes were successfully applied in the determination of these drugs in pharmaceutical preparations in four batches of different expiry dates. Statistical Student's t test and F test showed insignificant systematic error between the ion-selective electrode methods developed and a standard method. Comparison of the results obtained using the proposed electrodes with those found using a reference method showed that the ion-selective electrode technique is sensitive, reliable, and can be used with very good accuracy and high percentage recovery without pretreatment procedures of the samples to minimize interfering matrix effects. © 2007 Springer-Verlag.
Citation: Analytical and Bioanalytical Chemistry (2007), 389(6): 2019-2028
Issue Date: 2007
Keywords: Antiepileptic drugs
Membrane electrodes
Pharmaceutical analysis
ISSN: 16182642
Author Scopus IDs: 7403420647
Author Affiliations: Gupta, V.K., Department of Chemistry, Indian Institute of Technology-Roorkee, Roorkee 247667, India
Singh, A.K., Department of Chemistry, Indian Institute of Technology-Roorkee, Roorkee 247667, India
Gupta, B., Department of Chemistry, Indian Institute of Technology-Roorkee, Roorkee 247667, India
Funding Details: Acknowledgement B.G. gratefully acknowledges the Ministry of Human Resource Development, New Delhi, India, for financial support.
Corresponding Author: Gupta, V.K.; Department of Chemistry, Indian Institute of Technology-Roorkee, Roorkee 247667, India; email:
Appears in Collections:Journal Publications [CY]

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