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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/2998
Title: In vitro and in vivo studies on degradation of quinalphos in rats
Authors: Gupta B.
Rani M.
Salunke R.
Kumar R.
Published in: Journal of Hazardous Materials
Abstract: A pharmacokinetic in vitro and in vivo degradation study has been carried out in rat to evaluate the deleterious effects of exposure to quinalphos on a target population. Degradation of quinalphos in simulated gastric and intestinal phases has been investigated. The metabolic intermediates of quinalphos in serum and urine of albino rats at different time intervals were identified after dosing the animals with 5mgkg -1 body weight. All the samples were lyophilised, extracted and analysed by HPLC and GC-MS. The rate of degradation of quinalphos was accelerated in the presence of the enzymes pepsin and pancreatin contained in the gastric and intestinal simulations, respectively. Quinalphos oxon, O-ethyl-O-quinoxalin-2-yl phosphoric acid, 2-hydroxy quinoxaline and ethyl phosphoric acid are among the important metabolites identified both in in vitro and in vivo investigations. In simulated in vitro study some isomerised derivatives which were missing in the blood and urine of treated animals were identified. This could possibly be either due to non-formation or faster decay of the isomerised derivatives because of slightly different conditions prevailing in the two cases. The results also indicate that the metabolites, 2-hydroxy quinoxaline and oxon, which are more toxic than the parent compound, seem to persist for a longer time. © 2012.
Citation: Journal of Hazardous Materials (2012), 213-214(): 285-291
URI: https://doi.org/10.1016/j.jhazmat.2012.01.089
http://repository.iitr.ac.in/handle/123456789/2998
Issue Date: 2012
Keywords: 2-Hydroxyquinoxaline
Degradation products
In vitro
In vivo
Quinalphos
ISSN: 3043894
Author Scopus IDs: 7402195167
56363756800
23991735800
55492126800
Author Affiliations: Gupta, B., Analytical Laboratory, Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee, 247667(U.K.), India
Rani, M., Analytical Laboratory, Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee, 247667(U.K.), India
Salunke, R., Molecular Endocrinology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, 247667(U.K.), India
Kumar, R., Analytical Laboratory, Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee, 247667(U.K.), India
Funding Details: The authors would like to acknowledge Prof. S.N. Tandon (Retired), Former Faculty, Indian Institute of Technology Roorkee, Roorkee –247667(U.K.) India for his valuable suggestions and to Dr. Partha Roy, Associate Professor, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee – 247667(U.K.) India for providing laboratory facility. The authors are highly grateful to Council of Scientific and Industrial Research (CSIR) , India for funding the scheme.
Corresponding Author: Gupta, B.; Analytical Laboratory, Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee, 247667(U.K.), India; email: bvgcyfcy@iitr.ernet.in
Appears in Collections:Journal Publications [CY]

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