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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/2974
Title: Modification in the expression of mre11/rad50/nbs1 complex in low dose irradiated human lymphocytes
Authors: Singh S.
Bala M.
Kumar R.
Kumar A.
Dhiman S.C.
Published in: Dose-Response
Abstract: Despite the fact that high doses of radiation are detrimental, low dose radiation (LDR) often protects the organism against a subsequent exposure of lethal doses of radiation. Present study was undertaken to understand the role of Mre11, Rad50 and Nbs1 genes in the low dose radio-adapted human peripheral blood mononuclear cells (PBMCs). Optimum time interval between low dose (0.07 Gy) and high dose (5.0 Gy) of 60Co-y-radi-ation was observed to be 5.0 hours, at which PBMCs showed maximum LDR induced resistance (RIR). At cytogenetic level, micronuclei frequency was found to be reduced in LDR pre-irradiated PBMCs subsequently exposed to high dose radiation (HDR) as compared to controls. At transcriptional level, with reference to sham-irradiated cells significantly (p?0.05) altered expression of Mre11, Rad50 and Nbs1 genes was observed in low dose irradiated cells. At protein level, Mre11, Rad50 and Nbs1 were enhanced significantly (p?0.05) in low dose pre-irradiated cells subsequently exposed to high dose of radiation as compared to only high dose irradiated cells. Transcriptional as well as translational modulation in the expression of MRN complex components upon low dose irradiation may confer its participation in repair pathways, resulting in induced resistance.
Citation: Dose-Response (2009), 7(3): 193-207
URI: https://doi.org/10.2203/dose-response.09-001
http://repository.iitr.ac.in/handle/123456789/2974
Issue Date: 2009
Keywords: Gene expression
Immuno-blotting.
Ionizing radiation
Micronuclei
MRN complex
Radioadaptive response
ISSN: 15593258
Author Scopus IDs: 56010084800
7006891808
55553737050
7408043275
57197234997
Author Affiliations: Singh, S., Department of Zoology, M. S. (PG) College, Saharanpur-247 001, India
Bala, M., Division of Radiation Biosciences, Institute of Nuclear Medicine and Allied Sciences, Brig SK Mazumdar Marg, Delhi-110 054, India
Kumar, R., Division of Radiation Biosciences, Institute of Nuclear Medicine and Allied Sciences, Brig SK Mazumdar Marg, Delhi-110 054, India
Kumar, A., Division of Radiation Biosciences, Institute of Nuclear Medicine and Allied Sciences, Brig SK Mazumdar Marg, Delhi-110 054, India
Dhiman, S.C., Department of Zoology, M. S. (PG) College, Saharanpur-247 001, India
Corresponding Author: Singh, S.; Department of Zoology, M. S. (PG) College, Saharanpur-247 001, India; email: somuatar@gmail.com
Appears in Collections:Journal Publications [CY]

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