Skip navigation
Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/26967
Title: Crystallization and structure determination of goat lactoferrin at 4.0 Å resolution: A new form of packing in lactoferrins with a high solvent content in crystals
Authors: Kumar, Pravindra R.Manish
Yadav S.
Singh T.P.
Published in: Indian Journal of Biochemistry and Biophysics
Abstract: Lactoferrin was purified from fresh samples of goat colostrums, saturated with Fe 3+ and CO 3 2- ions and crystallized by microdialysis method. The crystals belong to orthorhombic space group P2 12 12 1 with a=104.6 Å, b=153.8 Å, c=155.1 Å and Z=4. The quality of crystals was poor, thus the intensity data were restricted to 4.0 Å resolution only. The structure was determined by molecular replacement method using diferric buffalo lactoferrin as a model. The solution clearly indicated the presence of one molecule in the asymmetric unit, which corresponds to a Vm value of 7.1 Å 3/Da. The structure was refined with stringent constraints to an R-factor of 0.246 using all the reflections 15,870 to 4.0 Å resolution. The overall structure of goat lactoferrin is essentially similar to those of buffalo and bovine lactoferrins. However, the iron-binding environment in goat lactoferrin is somewhat different, in which 2 CO 3 2- ions have low occupancies. The solvent content of approximately 84% was very high in the present case which explains the fragility of the crystals of goat lactoferrin. In a way, it is very surprising that the crystals grow at all, although crystals with solvent as high as 89% have been reported.
Citation: Indian Journal of Biochemistry and Biophysics, 39(1): 16-21
URI: http://repository.iitr.ac.in/handle/123456789/26967
Issue Date: 2002
Keywords: lactoferricin
lactoferrin
solvent
article
buffalo
chemical structure
controlled study
crystal
crystallization
goat
microdialysis
nonhuman
protein purification
sampling
structure analysis
X ray crystallography
Animals
Anions
Binding Sites
Colostrum
Crystallization
Crystallography, X-Ray
Goats
Iron
Lactoferrin
Metals
Models, Molecular
Molecular Conformation
Protein Conformation
Protein Structure, Secondary
Solvents
Bovinae
Bubalus
Capra hircus
ISSN: 3011208
Author Scopus IDs: 55064809000
37162551900
57218945130
Author Affiliations: Kumar, P., Department of Biophysics, All India Inst. of Medical Sciences, New Delhi 110 029, India
Yadav, S., Department of Biophysics, All India Inst. of Medical Sciences, New Delhi 110 029, India
Singh, T.P., Department of Biophysics, All India Inst. of Medical Sciences, New Delhi 110 029, India
Funding Details: 
Corresponding Author: Singh, T.P.; Department of Biophysics, , New Delhi 110 029, India; email: tps@aiims.aiims.ac.in
Appears in Collections:Journal Publications [BT]

Files in This Item:
There are no files associated with this item.
Show full item record


Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.