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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/26956
Title: Anaerobic crystallization and initial X-ray diffraction data of biphenyl 2,3-dioxygenase from Burkholderia xenovorans LB400: Addition of agarose improved the quality of the crystals
Authors: Kumar, Pravindra R.Manish
Gómez-Gil L.
Mohammadi M.
Sylvestre M.
Eltis L.D.
Bolin J.T.
Published in: Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Abstract: Biphenyl 2,3-dioxygenase (BPDO; EC 1.14.12.18) catalyzes the initial step in the degradation of biphenyl and some polychlorinated biphenyls (PCBs). BPDOLB400, the terminal dioxygenase component from Burkholderia xenovorans LB400, a proteobacterial species that degrades a broad range of PCBs, has been crystallized under anaerobic conditions by sitting-drop vapour diffusion. Initial crystals obtained using various polyethylene glycols as precipitating agents diffracted to very low resolution (8 Å) and the recorded reflections were diffuse and poorly shaped. The quality of the crystals was significantly improved by the addition of 0.2% agarose to the crystallization cocktail. In the presence of agarose, wild-type BPDOLB400 crystals that diffracted to 2.4 Å resolution grew in space group P1. Crystals of the BPDOP4 and BPDORR41 variants of BPDOLB400 grew in space group P21. © 2011 International Union of Crystallography. All rights reserved.
Citation: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 67(1): 59-63
URI: https://doi.org/10.1107/S1744309110043393
http://repository.iitr.ac.in/handle/123456789/26956
Issue Date: 2011
Keywords: agarose gel
biphenyl 2,3-dioxygenase
Burkholderia xenovorans LB400
ISSN: 17443091
Author Scopus IDs: 55064809000
18233632900
23390544500
7005984229
7003863696
57197844041
Author Affiliations: Kumar, P., Department of Biological Sciences, Center for Cancer Research, Purdue University, West Lafayette, IN, United States, Department of Biotechnology, Indian Institute of Technology, Roorkee, India
Gómez-Gil, L., Department of Microbiology, Life Sciences Institute, University of British Columbia, Vancouver, BC V6T 1Z3, Canada
Mohammadi, M., Institut National de Recherche Scientifique (INRS-Institut Armand-Frappier), Laval, QC H7V 1B7, Canada
Sylvestre, M., Institut National de Recherche Scientifique (INRS-Institut Armand-Frappier), Laval, QC H7V 1B7, Canada
Eltis, L.D., Department of Microbiology, Life Sciences Institute, University of British Columbia, Vancouver, BC V6T 1Z3, Canada
Bolin, J.T., Department of Biological Sciences, Center for Cancer Research, Purdue University, West Lafayette, IN, United States
Funding Details: 
Corresponding Author: Kumar, P.; Department of Biological Sciences, , West Lafayette, IN, United States; email: kumarfbs@iitr.ernet.in
Appears in Collections:Journal Publications [BT]

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