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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/24751
Title: Modified tamarind kernel polysaccharide-based matrix alters neuro-keratinocyte cross-talk and serves as a suitable scaffold for skin tissue engineering
Authors: Choudhury P.
Chawla S.
Agarwal S.
Singh A.
Nayak A.
Kumar A.
Maji, Pradip K.
Goswami C.
Goswami L.
Published in: Materials Science and Engineering C
Abstract: Advanced technologies like skin tissue engineering are requisite of various disorders where artificially synthesized materials need to be used as a scaffold in vivo, which in turn can allow the formation of functional skin and epidermal layer with all biological sensory functions. In this work, we present a set of hydrogels which have been synthesized by the method utilizing radical polymerization of a natural polymer extracted from kernel of Tamarindus indica, commonly known as Tamarind Kernel Powder (TKP) modified by utilizing the monomer acrylic acid (AA) in different mole ratios. These materials are termed as TKP: AA hydrogels and characterized by Atomic Force Microscopy (AFM), surface charge, and particle size distribution using Dynamic Light Scattering measurements. These materials are biocompatible with mouse dermal fibroblasts (NIH- 3T3) and human skin keratinocytes (HaCaT), as confirmed by MTT and biocompatibility assays. These TKP: AA hydrogels do not induce unwanted ROS signaling as confirmed by mitochondrial functionality determined by DCFDA staining, Mitosox imaging, and measuring the ATP levels. We demonstrate that in the co-culture system, TKP: AA allows the establishment of proper neuro-keratinocyte contact formation, suggesting that this hydrogel can be suitable for developing skin with sensory functions. Skin corrosion analysis on SD rats confirms that TKP: AA is appropriate for in vivo applications as well. This is further confirmed by in vivo compatibility and toxicity studies, including hemocompatibility and histopathology of liver and kidney upon direct introduction of hydrogel into the body. We propose that TKP: AA (1: 5) offers a suitable surface for skin tissue engineering with sensory functions applicable in vitro, in vivo, and ex vivo. These findings may have broad biomedical and clinical importance. © 2020 Elsevier B.V.
Citation: Materials Science and Engineering C, 121
URI: https://doi.org/10.1016/j.msec.2020.111779
http://repository.iitr.ac.in/handle/123456789/24751
Issue Date: 2021
Publisher: Elsevier Ltd
Keywords: ATP
Hydrogel
Keratinocyte
Peripheral neuron
Skin
ISSN: 9284931
Author Scopus IDs: 57191596553
55839292300
57221269679
57201123882
57221264756
57211505695
56249287900
8236317400
36887214600
Author Affiliations: Choudhury, P., School of Biotechnology, Kalinga Institute of Industrial Technology, Patia, Bhubaneswar, 751024, India
Chawla, S., School of Biological Sciences, National Institute of Science and Educational Research, Homi Bhabha National Institute, Khorda, Jatni, 752050, India
Agarwal, S., School of Biotechnology, Kalinga Institute of Industrial Technology, Patia, Bhubaneswar, 751024, India
Singh, A., School of Biotechnology, Kalinga Institute of Industrial Technology, Patia, Bhubaneswar, 751024, India
Nayak, A., School of Biotechnology, Kalinga Institute of Industrial Technology, Patia, Bhubaneswar, 751024, India
Kumar, A., School of Biological Sciences, National Institute of Science and Educational Research, Homi Bhabha National Institute, Khorda, Jatni, 752050, India
Maji, P.K., Department of Polymer & Process Engineering, Indian Institute of Technology Roorkee, Saharanpur Campus, Saharanpur, 247001, India
Goswami, C., School of Biological Sciences, National Institute of Science and Educational Research, Homi Bhabha National Institute, Khorda, Jatni, 752050, India
Goswami, L., School of Biotechnology, Kalinga Institute of Industrial Technology, Patia, Bhubaneswar, 751024, India, School of Chemical Technology, Kalinga Institute of Industrial Technology, Patia, Bhubaneswar, 751024, India
Funding Details: Authors acknowledge Hindustan Gums and Chemicals ltd, India, for providing Tamarind Kernel Powder. Infrastructure support from the KIIT School of Biotechnology is acknowledged. Intramural supports from NISER are appreciated and acknowledged. Imaging and animal facilities of NISER are acknowledged. PC is supported financially by the DST- INSPIRE fellowship ( IF 140011 ), New Delhi, India. CG and LG acknowledge support from Ram Prasad Sahu, and all other present and former lab members. Department of Science and Technology, Ministry of Science and Technology, India, डीएसटी; Department of Science and Technology, Government of West Bengal, DST: IF 140011; National Institute of Science Education and Research, NISER
Corresponding Author: Goswami, L.; School of Biotechnology, Patia, India; email: lgoswami@kiitbiotech.ac.in
Appears in Collections:Journal Publications [PE]

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