Skip navigation
Please use this identifier to cite or link to this item:
Title: Gelatin-polycaprolactone-nanohydroxyapatite electrospun nanocomposite scaffold for bone tissue engineering
Authors: Gautam S.
Sharma C.
Purohit S.D.
Singh H.
Dinda A.K.
Potdar P.D.
Chou C.-F.
Mishra, Narayan Chandra
Published in: Materials Science and Engineering C
Abstract: Bone injuries and fractures generally take a long period to heal itself. To address this problem, bone tissue engineering (BTE) has gained significant research impetus. Among the several techniques used for scaffold fabrication, electrospinning ought to be the most promising technique for the development of the nanostructured scaffolds. The present study was carried out to fabricate an electrospun nanocomposite scaffold for BTE by using gelatin, polycaprolactone (PCL), and nanohydroxyapatite (nHAp). To prepare Gelatin-PCL-nHAp nanocomposite scaffold: Gelatin-PCL blend was electrospun and then treated with nHAp (1 wt%) for different time periods. The fabricated nanocomposite scaffold was analysed by field emission scanning electron microscopy (FESEM) to determine the fiber diameter and evaluate the fiber morphology. The Gelatin-PCL-nHAp nanocomposite scaffold-20 min exhibited the average fiber diameter of 615±269 nm and average pore size 4.7±1.04 μm, and also revealed the presence of nHAp particles over the Gelatin-PCL scaffold surface. Further, X-ray diffraction (XRD), Fourier Transform Infrared (FTIR) spectroscopy and thermogravimetric (TG) analysis also indicated the deposition of nHAp over the Gelatin-PCL scaffold surface. MTT assay and DNA quantification showed good viability and significant proliferation of human osteoblasts on Gelatin-PCL-nHAp nanocomposite scaffold. Moreover, cell-scaffold constructs illustrated efficient cellular attachment and adequately spread cells, and it also depicts characteristic polygonal morphology of osteoblasts over the Gelatin-PCL-nHAp nanocomposite scaffold. Thus, the results of in-vitro analysis of electrospun nanocomposite scaffold suggest that the Gelatin-PCL-nHAp scaffold can be a potential candidate for BTE applications. © 2020 Elsevier B.V.
Citation: Materials Science and Engineering C, 119
Issue Date: 2021
Publisher: Elsevier Ltd
Keywords: Bone tissue engineering
ISSN: 9284931
Author Scopus IDs: 57213372885
Author Affiliations: Gautam, S., Department of Molecular Biology & Genetic Engineering, CBSH, G. B. Pant University of Agriculture & Technology, Pantnagar, India
Sharma, C., Avantha Centre for Industrial Research and Development, Paper Mill Campus, Yamuna Nagar, Haryana, India
Purohit, S.D., Department of Polymer & Process Engineering, India Institute of Technology, Roorkee, India
Singh, H., Department of Polymer & Process Engineering, India Institute of Technology, Roorkee, India
Dinda, A.K., Department of Pathology, All India Institute of Medical Science, New Delhi, India
Potdar, P.D., Department of Molecular Medicine & Biology, Jaslok Hospital & Research Centre, Mumbai, India
Chou, C.-F., Institute of Physics, Academia Sinica, Taipei, Taiwan
Mishra, N.C., Department of Polymer & Process Engineering, India Institute of Technology, Roorkee, India
Funding Details: The authors are grateful to Indian Institute of Technology, Roorkee for providing experiment facility of scaffold synthesis and characterization. The financial assistance from Ministry of Human Resource Development (MHRD) India, is gratefully acknowledged. We also thankfully acknowledge the management of Jaslok Hospital and Research Centre, Mumbai for providing the facility of cell culture, and especially Mr. Sachin Ramdas Chaugule, Research Assistant, Department of Molecular Medicine and Biology to carry out the biocompatibility experiments. Sneh Gautam: Conceptualization, Methodology, investigation, Writing- Original draft preparation. Chhavi Sharma: Investigation, Reviewing and Editing. Shiv Dutt Purohit: Visualization, Investigation, Writing- Reviewing and Editing. Hemat Singh: Reviewing and Editing. Amit Kumar Dinda: Investigation, Methodology for biocompatibility. Pravin D Potdar: Biocompatibility evaluation and characterization. Chia-Fu Chou: Methodology, Investigation. Narayan Chandra Mishra: Supervision, Resources, Funding acquisition. Ministry of Human Resource Development, MHRD
Corresponding Author: Mishra, N.C.; Department of Polymer & Process Engineering, Saharanpur Campus, India; email:
Appears in Collections:Journal Publications [PE]

Files in This Item:
There are no files associated with this item.
Show full item record

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.