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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/2306
Title: Evaluation of chitosan and their self-assembled nanoparticles with pDNA for the application in gene therapy
Authors: Sarkar K.
Srivastava R.
Chatterji U.
Kundu, Patit Paban
Published in: Journal of Applied Polymer Science
Abstract: The molecular weight (MW) of chitosan (CS) was determined by viscometric method (using Mark-Houwink equation) as well as by gel permeation chromatography, and the degree of deacetylation (DDA) of CS was measured by potentiometric titration method and Gran-type linearization method. The values of DDA were obtained â 83% (by potentiometric titration method) and â 86% (by Gran-type linearization method). The self-assembled nanoparticles of CS/plasmid DNA (pDNA) complex were prepared by varying the concentration of CS. The formation of CS/pDNA complex was confirmed by 0.8% agarose gel electrophoresis and the particle size of the self-assembled nanoparticle was determined by dynamic light scattering, atomic force microscopy and scanning electron microscopy. The stability of CS/pDNA complexes was determined by turbidity test with the help of UV-Vis spectroscopy. The effect of ionic strength on the complexes was also observed by means of fluorescence spectroscopy. The cytotoxicity of CS on the HeLa cell line was observed by absorbance of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and showed that CS has lower cytotoxicity in HeLa cells compared with that of poly (L-lysine) in 293T cells. © 2011 Wiley Periodicals, Inc.
Citation: Journal of Applied Polymer Science (2011), 121(4): 2239-2249
URI: https://doi.org/10.1002/app.33832
http://repository.iitr.ac.in/handle/123456789/2306
Issue Date: 2011
Keywords: chitosan
DNase I
dynamic light scattering
nanoparticles
ISSN: 218995
Author Scopus IDs: 35771416500
37666173900
6602466768
35475516300
Author Affiliations: Sarkar, K., Department of Polymer Science and Technology, University of Calcutta, Kolkata-9, India
Srivastava, R., Department of Polymer Science and Technology, University of Calcutta, Kolkata-9, India
Chatterji, U., Department of Zoology, University of Calcutta, Kolkata-9, India
Kundu, P.P., Department of Polymer Science and Technology, University of Calcutta, Kolkata-9, India
Corresponding Author: Kundu, P. P.; Department of Polymer Science and Technology, University of Calcutta, Kolkata-9, India; email: ppk923@yahoo.com
Appears in Collections:Journal Publications [CH]

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