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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/2302
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dc.contributor.authorSarkar K.-
dc.contributor.authorKundu, Patit Paban-
dc.date.accessioned2020-10-06T14:09:03Z-
dc.date.available2020-10-06T14:09:03Z-
dc.date.issued2012-
dc.identifier.citationInternational Journal of Biological Macromolecules (2012), 51(5): 859-867-
dc.identifier.issn1418130-
dc.identifier.other22800728-
dc.identifier.urihttps://doi.org/10.1016/j.ijbiomac.2012.06.038-
dc.identifier.urihttp://repository.iitr.ac.in/handle/123456789/2302-
dc.description.abstractLow molecular weight N-maleated chitosan-graft-PAMAM (polyamidoamine) copolymer was prepared through N-maleated chitosan (NMC) by Michael type addition reaction to enhance its solubility in water as well as its cationic character for enhancement of DNA complexation. FTIR, 1H NMR, XRD and GPC were used to characterize the graft copolymers. The copolymer showed better DNA complexation ability at low N/P ratio than that of chitosan due to increased surface charge density by the incorporation of PAMAM molecule on to chitosan backbone. The copolymer can effectively protect the DNA toward anionic surfactant. In vitro release study showed efficient DNA release occurred at physiological pH (pH 7.4). In vitro cell cytotoxicity test indicated toward less cytotoxicity of NMC-graft-PAMAM copolymers compared to that of 25kDa PEI. Thus, the synthesized NMC-graft-PAMAM copolymers have great potential of finding application in drug and gene delivery. © 2012 Elsevier B.V.-
dc.language.isoen_US-
dc.relation.ispartofInternational Journal of Biological Macromolecules-
dc.subjectChitosan-
dc.subjectDNA complex-
dc.subjectGraft copolymer-
dc.subjectIn vitro cytotoxicity-
dc.subjectPAMAM dendrimer-
dc.titlePreparation of low molecular weight N-maleated chitosan-graft-PAMAM copolymer for enhanced DNA complexation-
dc.typeArticle-
dc.scopusid35771416500-
dc.scopusid35475516300-
dc.affiliationSarkar, K., Department of Polymer Science and Technology, University of Calcutta, 92 A.P.C. Road, Kolkata 9, India-
dc.affiliationKundu, P.P., Department of Polymer Science and Technology, University of Calcutta, 92 A.P.C. Road, Kolkata 9, India-
dc.description.fundingThis work was financially supported by the University Grant Commission (UGC), India under the Rajiv Gandhi National Fellowship. The authors also acknowledge Dr. Gopal Chackraborty, Dr. B.C. Guha Centre for Genetic Engineering and Biotechnology, University of Calcutta for cytotoxicity measurements.-
dc.description.correspondingauthorKundu, P.P.; Department of Polymer Science and Technology, University of Calcutta, 92 A.P.C. Road, Kolkata 9, India; email: ppk922@yahoo.com-
Appears in Collections:Journal Publications [CH]

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