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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/2302
Title: Preparation of low molecular weight N-maleated chitosan-graft-PAMAM copolymer for enhanced DNA complexation
Authors: Sarkar K.
Kundu, Patit Paban
Published in: International Journal of Biological Macromolecules
Abstract: Low molecular weight N-maleated chitosan-graft-PAMAM (polyamidoamine) copolymer was prepared through N-maleated chitosan (NMC) by Michael type addition reaction to enhance its solubility in water as well as its cationic character for enhancement of DNA complexation. FTIR, 1H NMR, XRD and GPC were used to characterize the graft copolymers. The copolymer showed better DNA complexation ability at low N/P ratio than that of chitosan due to increased surface charge density by the incorporation of PAMAM molecule on to chitosan backbone. The copolymer can effectively protect the DNA toward anionic surfactant. In vitro release study showed efficient DNA release occurred at physiological pH (pH 7.4). In vitro cell cytotoxicity test indicated toward less cytotoxicity of NMC-graft-PAMAM copolymers compared to that of 25kDa PEI. Thus, the synthesized NMC-graft-PAMAM copolymers have great potential of finding application in drug and gene delivery. © 2012 Elsevier B.V.
Citation: International Journal of Biological Macromolecules (2012), 51(5): 859-867
URI: https://doi.org/10.1016/j.ijbiomac.2012.06.038
http://repository.iitr.ac.in/handle/123456789/2302
Issue Date: 2012
Keywords: Chitosan
DNA complex
Graft copolymer
In vitro cytotoxicity
PAMAM dendrimer
ISSN: 1418130
Author Scopus IDs: 35771416500
35475516300
Author Affiliations: Sarkar, K., Department of Polymer Science and Technology, University of Calcutta, 92 A.P.C. Road, Kolkata 9, India
Kundu, P.P., Department of Polymer Science and Technology, University of Calcutta, 92 A.P.C. Road, Kolkata 9, India
Funding Details: This work was financially supported by the University Grant Commission (UGC), India under the Rajiv Gandhi National Fellowship. The authors also acknowledge Dr. Gopal Chackraborty, Dr. B.C. Guha Centre for Genetic Engineering and Biotechnology, University of Calcutta for cytotoxicity measurements.
Corresponding Author: Kundu, P.P.; Department of Polymer Science and Technology, University of Calcutta, 92 A.P.C. Road, Kolkata 9, India; email: ppk922@yahoo.com
Appears in Collections:Journal Publications [CH]

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