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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/2301
Title: Oral insulin delivery by self-assembled chitosan nanoparticles: In vitro and in vivo studies in diabetic animal model
Authors: Mukhopadhyay P.
Sarkar K.
Chakraborty M.
Bhattacharya S.
Mishra R.
Kundu, Patit Paban
Published in: Materials Science and Engineering C
Abstract: We have developed self-assembled chitosan/insulin nanoparticles for successful oral insulin delivery. The main purpose of our study is to prepare chitosan/insulin nanoparticles by self-assembly method, to characterize them and to evaluate their efficiency in vivo diabetic model. The size and morphology of the nanoparticles were analyzed by dynamic light scattering (DLS), atomic force microscopy (AFM) and scanning electron microscopy (SEM). The average particle size ranged from 200 to 550 nm, with almost spherical or sub spherical shape. An average insulin encapsulation within the nanoparticles was ~ 85%. In vitro release study showed that the nanoparticles were also efficient in retaining good amount of insulin in simulated gastric condition, while significant amount of insulin release was noticed in simulated intestinal condition. The oral administrations of chitosan/insulin nanoparticles were effective in lowering the blood glucose level of alloxan-induced diabetic mice. Thus, self-assembled chitosan/insulin nanoparticles show promising effects as potential insulin carrier system in animal models. © 2012 Elsevier B.V.
Citation: Materials Science and Engineering C (2013), 33(1): 376-382
URI: https://doi.org/10.1016/j.msec.2012.09.001
http://repository.iitr.ac.in/handle/123456789/2301
Issue Date: 2013
Keywords: Chitosan
Insulin
Nanoparticles
Oral delivery
Self-assembly
ISSN: 9284931
Author Scopus IDs: 55235955100
35771416500
35809824000
57072241500
25643271200
35475516300
Author Affiliations: Mukhopadhyay, P., Department of Polymer Science and Technology, University of Calcutta, 92, A.P.C. Road, Kolkata-700009, India
Sarkar, K., Department of Polymer Science and Technology, University of Calcutta, 92, A.P.C. Road, Kolkata-700009, India
Chakraborty, M., Department of Physiology, University of Calcutta, 92, A.P.C. Road, Kolkata-700009, India
Bhattacharya, S., Department of Physiology, University of Calcutta, 92, A.P.C. Road, Kolkata-700009, India
Mishra, R., Department of Physiology, University of Calcutta, 92, A.P.C. Road, Kolkata-700009, India
Kundu, P.P., Department of Polymer Science and Technology, University of Calcutta, 92, A.P.C. Road, Kolkata-700009, India
Corresponding Author: Kundu, P.P.; Department of Polymer Science and Technology, University of Calcutta, 92, A.P.C. Road, Kolkata-700009, India; email: ppk923@yahoo.com
Appears in Collections:Journal Publications [CH]

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