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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/2299
Title: Blood compatible N-maleyl chitosan-graft-PAMAM copolymer for enhanced gene transfection
Authors: Sarkar K.
Chatterjee A.
Chakraborti G.
Kundu P.P.
Published in: Carbohydrate Polymers
Abstract: To improve transfection efficiency, we prepared N-maleyl chitosan-graft-polyamidoamine (NMCTS-graft- PAMAM) copolymer. Self-assembled NMCTS-graft-PAMAM/pDNA complexes were prepared by complex coacervation method at different N/P (nitrogen to phosphate ratio) ratios. The copolymer effectively formed complexes with pDNA at lower N/P ratio (N/P ratio 1.0) than that of unmodified chitosan (N/P ratio 2.0) and the complexes were spherical with particle size of 100-150 nm. The copolymer showed significant protection of DNA from nuclease attack with lower toxicity against HeLa cell. The copolymer also showed no noticeable hemolytic effects up to 10 mg/mL indicating no detectable disturbance of the red blood cell membranes. The transfection efficiency of the copolymer was increased significantly compared to that of chitosan and reached up to 36 ± 2% at N/P ratio 7.0 which was higher than that of PEI (30 ± 3% at N/P ratio 10). Therefore, the copolymer may be a strong alternative candidate as effective nonviral vector. © 2013 Elsevier Ltd. All rights reserved.
Citation: Carbohydrate Polymers (2013), 98(1): 596-606
URI: https://doi.org/10.1016/j.carbpol.2013.06.035
http://repository.iitr.ac.in/handle/123456789/2299
Issue Date: 2013
Publisher: Elsevier Ltd
Keywords: Chitosan
Hemolysis
In vitro transfection
Nanoparticle
PAMAM dendrimer
Self-assembled
ISSN: 1448617
Author Scopus IDs: 35771416500
57199867967
54400863500
35475516300
Author Affiliations: Sarkar, K., Department of Polymer Science and Technology, University of Calcutta, 92 A.P.C. Road, Kolkata 700009, India
Chatterjee, A., Department of Biotechnology, Dr. B.C. Guha Centre for Genetic Engineering, University of Calcutta, 35, Ballygunje Circular Road, Kolkata 700019, India
Chakraborti, G., Department of Biotechnology, Dr. B.C. Guha Centre for Genetic Engineering, University of Calcutta, 35, Ballygunje Circular Road, Kolkata 700019, India
Kundu, P.P., Department of Polymer Science and Technology, University of Calcutta, 92 A.P.C. Road, Kolkata 700009, India
Funding Details: This work was financially supported by the University Grant Commission (UGC) , India under the Rajiv Gandhi National Fellowship and AICTE (RPS).
Corresponding Author: Kundu, P.P.; Department of Polymer Science and Technology, University of Calcutta, 92 A.P.C. Road, Kolkata 700009, India; email: ppk923@yahoo.com
Appears in Collections:Journal Publications [CH]

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