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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/2297
Title: Preparation of low toxic fluorescent chitosan-graft-polyethyleneimine copolymer for gene carrier
Authors: Sarkar K.
Debnath M.
Kundu, Patit Paban
Published in: Carbohydrate Polymers
Abstract: Fluorescent chitosan-graft-polyethyleneimine (PEI) copolymer was prepared by incorporating PEI molecule onto chitosan backbone through naphthalimide moiety by simple substitution reaction. 4-Bromo-1,8-naphthalic anhydride was used as fluorescent probe due to its strong fluorescence and good photo-stability property and the presence of a fine tunable bromide functional group in the naphthalimide ring, in this work. The copolymer was characterized by FTIR, elemental analysis and XRD. The fluorescence property of the copolymer was determined by UV-vis spectrometer and spectrofluorometer. The effects of pH and temperature on fluorescence property of the copolymer were also studied. The graft copolymer with degree of substitution 37.6 of PEI onto chitosan showed better complexation ability with DNA at comparatively low N/P (nitrogen to phosphate ratio) ratio 1.0 compared to that of chitosan (N/P ratio 2.0). The cytotoxicity of PEI largely decreased after grafting with chitosan and all the copolymers showed above 50% cell viability even at high polymer concentration (300 ?g/mL). Therefore, the prepared fluorescent chitosan-graft-PEI copolymer may be used as a biological marker as well as drug or gene carrier with low toxicity. © 2012 Elsevier Ltd.
Citation: Carbohydrate Polymers (2013), 92(2): 2048-2057
URI: https://doi.org/10.1016/j.carbpol.2012.11.067
http://repository.iitr.ac.in/handle/123456789/2297
Issue Date: 2013
Keywords: Chitosan
DNA complex
Fluorescence
Graft copolymer
In vitro toxicity
PEI
ISSN: 1448617
Author Scopus IDs: 35771416500
55537587300
35475516300
Author Affiliations: Sarkar, K., Department of Polymer Science and Technology, University of Calcutta, 92 A. P. C. Road, Kolkata-700009, India
Debnath, M., Department of Polymer Science and Technology, University of Calcutta, 92 A. P. C. Road, Kolkata-700009, India
Kundu, P.P., Department of Polymer Science and Technology, University of Calcutta, 92 A. P. C. Road, Kolkata-700009, India
Funding Details: This work was financially supported by the University Grant Commission (UGC), India under the Rajiv Gandhi National Fellowship.
Corresponding Author: Kundu, P.P.; Department of Polymer Science and Technology, University of Calcutta, 92 A. P. C. Road, Kolkata-700009, India; email: ppk923@yahoo.com
Appears in Collections:Journal Publications [CH]

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