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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/2286
Title: PAMAM conjugated chitosan through naphthalimide moiety for enhanced gene transfection efficiency
Authors: Sarkar K.
Kundu, Patit Paban
Published in: Carbohydrate Polymers
Abstract: Development of efficient and safe gene carrier is the main hurdle for successful gene therapy till date. Poor water solubility and low transfection efficiency of chitosan are the main drawbacks to be efficient gene carrier for successful gene therapy. In this work, PAMAM conjugated chitosan was prepared through naphthalimide moiety by simple substitution reaction. The synthesis of the chitosan conjugates was confirmed by FTIR, 1H NMR and XRD analyses. The conjugates showed enhanced DNA binding capability compared to that of unmodified chitosan. Moreover, the conjugates showed minimal cytotoxicity compared to that of polyethyleneimine (PEI, 25 kDa) and also showed good blood compatibility with negligible haemolysis. The transfection efficiency of the conjugate was significantly increased compared to that of unmodified chitosan and it also surpassed the transfection efficiency by PEI. Therefore, PAMAM conjugated chitosan can be used safely as alternate efficient gene delivery vector in gene therapy. © 2013 Elsevier Ltd. All rights reserved.
Citation: Carbohydrate Polymers (2013), 98(1): 495-504
URI: https://doi.org/10.1016/j.carbpol.2013.06.006
http://repository.iitr.ac.in/handle/123456789/2286
Issue Date: 2013
Keywords: Chitosan
Cytotoxicity
In vitro
PAMAM dendrimer
Transfection
ISSN: 1448617
Author Scopus IDs: 35771416500
35475516300
Author Affiliations: Sarkar, K., Department of Polymer Science and Technology, University of Calcutta, 92, A.P.C. Road, Kolkata 700009, West Bengal, India
Kundu, P.P., Department of Polymer Science and Technology, University of Calcutta, 92, A.P.C. Road, Kolkata 700009, West Bengal, India
Corresponding Author: Kundu, P.P.; Department of Polymer Science and Technology, University of Calcutta, 92, A.P.C. Road, Kolkata 700009, West Bengal, India; email: ppk923@yahoo.com
Appears in Collections:Journal Publications [CH]

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