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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/2231
Title: Synthesis of a carboxymethylated guar gum grafted polyethyleneimine copolymer as an efficient gene delivery vehicle
Authors: Jana P.
Sarkar K.
Mitra T.
Chatterjee A.
Gnanamani A.
Chakraborti G.
Kundu, Patit Paban
Published in: RSC Advances
Abstract: In the present study, a carboxymethylated guar gum-grafted-polyethyleneimine copolymer (CMGG-g-PEI) was synthesized and characterized by FT-IR, 1H NMR, XRD and zeta potential analyses. CMGG-g-PEI exhibited good binding ability with plasmid DNA (pDNA) and formed complexes with sizes ranging from 150 to 200 nm when the polymer/pDNA weight ratio was above 10:1. SEM analysis revealed a compact and spherical morphology of CMGG-g-PEI/pDNA complexes. The results from cytotoxicity and blood compatibility studies revealed the less toxic profile of CMGG-g-PEI. The in vitro gene transfection efficiency of the CMGG-g-PEI/pDNA complex was optimized in A549 cell and CMGG-g-PEI showed better transfection efficiency compared to the well-known standard polymer, polyethyleneimine (PEI). All the results suggested that the CMGG-g-PEI could find application as an alternative efficient gene delivery vehicle in the future. © 2016 The Royal Society of Chemistry.
Citation: RSC Advances (2016), 6(17): 13730-13741
URI: https://doi.org/10.1039/c5ra23447f
http://repository.iitr.ac.in/handle/123456789/2231
Issue Date: 2016
Publisher: Royal Society of Chemistry
ISSN: 20462069
Author Scopus IDs: 57102951300
35771416500
39863189900
57199867967
6602667464
54400863500
35475516300
Author Affiliations: Jana, P., Department of Polymer Science and Technology, University of Calcutta, 92 A.P.C Road, Kolkata, 700009, India
Sarkar, K., Department of Pharmaceutical Sciences, School of Pharmacy, University of PittsburghPA 15261, United States
Mitra, T., Department of Polymer Science and Technology, University of Calcutta, 92 A.P.C Road, Kolkata, 700009, India
Chatterjee, A., Department of Biotechnology, Dr. B.C. Guha Centre for Genetic Engineering, University of Calcutta, 35 Ballygung Circular Road, Kolkata, 700019, India
Gnanamani, A., Microbiology Division, CSIR-Central Leather Research Institute Adyar, Chennai, Tamil Nadu 600020, India
Chakraborti, G., Department of Biotechnology, Dr. B.C. Guha Centre for Genetic Engineering, University of Calcutta, 35 Ballygung Circular Road, Kolkata, 700019, India
Kundu, P.P., Department of Polymer Science and Technology, University of Calcutta, 92 A.P.C Road, Kolkata, 700009, India
Corresponding Author: Kundu, P.P.; Department of Polymer Science and Technology, University of Calcutta, 92 A.P.C Road, India; email: ppk923@yahoo.com
Appears in Collections:Journal Publications [CH]

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