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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/2210
Title: Preparation of guar gum scaffold film grafted with ethylenediamine and fish scale collagen, cross-linked with ceftazidime for wound healing application
Authors: Jana P.
Mitra T.
Selvaraj T.K.R.
Gnanamani A.
Kundu, Patit Paban
Published in: Carbohydrate Polymers
Abstract: Present study describes the synthesis of carboxymethyl guar gum (CMGG) from the native guar gum (GG) and the prepared CMGG is grafted with ethylenediamine (EDA) to form aminated CMGG. Then, fish scale collagen and aminated CMGG are cross-linked by ceftazidime drug through non- covalent ionic interaction. The resultant cross-linked film is subjected to the analysis of 1HNMR, ATR-FTIR, TGA, SEM and XRD. The TNBS results revealed that 45% of interaction between EDA and CMGG and 90–95% of Ceftazidime is released from aminated CMGG-Ceftazidime-Collagen (ACCC) film after 96 h of incubation at physiological pH. In vitro cell line studies reveal the biocompatibility of the cross-linked film and the antimicrobial studies display the growth inhibition against Staphylococcus aureus and Pseudomonas aeruginosa organisms. Overall, the study indicates that the incorporation of Ceftazidime into collagen and aminated CMGG can improve the functional property of aminated CMGG as well as collagen, leading to its biomedical applications. © 2016
Citation: Carbohydrate Polymers (2016), 153(): 573-581
URI: https://doi.org/10.1016/j.carbpol.2016.07.053
http://repository.iitr.ac.in/handle/123456789/2210
Issue Date: 2016
Publisher: Elsevier Ltd
Keywords: Ceftazidime cross-linker
Cyamopsis tetragonoloba seeds
Ethylenediamine
Fish scale collagen
Guar gum
ISSN: 1448617
Author Scopus IDs: 57102951300
39863189900
57190755530
6602667464
35475516300
Author Affiliations: Jana, P., Department of Polymer Science & Technology, University of Calcutta, University College of Science & Technology, 92, A.P.C Road, Kolkata, WestBengal 700009, India
Mitra, T., Department of Polymer Science & Technology, University of Calcutta, University College of Science & Technology, 92, A.P.C Road, Kolkata, WestBengal 700009, India
Selvaraj, T.K.R., Microbiology Division, CSIR-Central Leather Research Institute, Adyar, Chennai, Tamil Nadu 600020, India
Gnanamani, A., Microbiology Division, CSIR-Central Leather Research Institute, Adyar, Chennai, Tamil Nadu 600020, India
Kundu, P.P., Department of Polymer Science & Technology, University of Calcutta, University College of Science & Technology, 92, A.P.C Road, Kolkata, WestBengal 700009, India, Department of Chemical Engineering, Indian Institute of Technology, Roorkee, 247667, India
Funding Details: One of the authors Dr. Tapas Mitra acknowledges UGC, New Delhi, India for financial assistance provided in the form of Dr. D. S. Kothari Post Doctoral Fellowship. Appendix A
Corresponding Author: Mitra, T.; Department of Polymer Science & Technology, University of Calcutta, University College of Science & Technology, 92, A.P.C Road, India; email: tapasbiochem1@gmail.com
Appears in Collections:Journal Publications [CH]

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