A sustainable production method of mycelium biomass using an isolated fungal strain Phanerochaete chrysosporium (accession no: KY593186): Its exploitation in wound healing patch formation

Abstract

The present study reports the tuning of the culture condition for the synthesis of fibrous composite mycelium biomass having important physical properties by using an isolated novel fungal strain. The Internal Transcribed Spacer sequencing revealed that the isolated fungal strain has a close proximity with Phanerochaete chrysosporium (accession no: KY593186). The formation of the mycelium was confirmed by the appearance of white gelatinous pellicle in the liquid-air interface of liquid fermentation medium kept in an Erlenmeyer flask. The confirmation of the presence of carbohydrate in the obtained mycelium film was carried out using anthrone assay test. The prepared mycelium film was characterized using FTIR and FESEM analyses. After that, the mycelium film was loaded with the curcumin, showing significant antimicrobial activity against both Gram-positive and Gram-negative bacteria. In- vitro wound healing assay of the curcumin-loaded mycelium film reveals the potential of the composition for wound closure in the scratched cells. © 2018