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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/2179
Title: Cloning and expression of ?-amylase in E. coli: genesis of a superior biocatalyst for substrate-specific MFC
Authors: Nandy A.
Jana S.
Khamrai M.
Kumar V.
Mukherjee S.
Bhattacharyya A.
Kundu, Patit Paban
Published in: International Journal of Green Energy
Abstract: One of the most practical approaches for establishing a successful microbial fuel cell (MFC) is to fasten the oxidation rate of the substrate by the microorganisms to get quick paced electron transfer between microbes and electrode. A genetically modified Escherichiacoli, overexpressing ?-amylase, is constructed and applied as biocatalyst in MFC using starch as substrate. The results are compared with nonrecombinant, native E.coli. The results show better performance for the MFC containing the recombinant strain demonstrated by higher power density (PD), lower resistance, and significant electrochemical activity. Maximum PD has been recorded as 279.04 mW m ?2 compared to 120.33 Mw m ?2 for the MFC operated with nonrecombinant E.coli. The impedance results also suggest the effectiveness of the recombinant strain by lowering the internal resistance by more than half order as compared to the nonrecombinant one. These results affirm that the engineered strain can be used as a superior biocatalyst in contrast to the native strain and by using the technique of genetic alteration; gene of interest can be inserted based on the substrate to be treated. So, this work gives a useful insight for accomplishing successful MFC operation with the use of bacterial stains engineered at the molecular level. © 2019, © 2019 Taylor & Francis Group, LLC.
Citation: International Journal of Green Energy (2019), 16(4): 309-316
URI: https://doi.org/10.1080/15435075.2019.1566135
http://repository.iitr.ac.in/handle/123456789/2179
Issue Date: 2019
Publisher: Taylor and Francis Inc.
Keywords: biocatalyst
genetic modification
Microbial fuel cell
power density
sustainable
ISSN: 15435075
Author Scopus IDs: 55813019000
55780007100
56641668300
57197697614
56092079800
57201696278
35475516300
Author Affiliations: Nandy, A., Department of Polymer Science and Technology, University of Calcutta, Kolkata, India
Jana, S., Department of Zoology, University of Calcutta, Kolkata, India
Khamrai, M., Department of Polymer Science and Technology, University of Calcutta, Kolkata, India
Kumar, V., Department of Civil and Environmental Engineering, IIT Patna, Patna, India
Mukherjee, S., Department of Polymer Science and Technology, University of Calcutta, Kolkata, India
Bhattacharyya, A., Department of Zoology, University of Calcutta, Kolkata, India
Kundu, P.P., Department of Polymer Science and Technology, University of Calcutta, Kolkata, India, Department of Chemical Engineering, IIT Roorkee, Roorkee, India
Funding Details: The financial support in the form of Senior Research Fellowship [09/ 028(0920)/2014-EMR-I] provided by the Council of Scientific and Industrial Research, India, is gratefully acknowledged by Mrs Arpita Nandy.Vikash Kumar also acknowledges the financial support from DST-SERB for National Postdoctoral Fellowship [PDF/2016/001018]. ;This work was supported by the Department of Science and Technology (DST) Science and Engineering Research Board (SERB) [PDF/2016/ 001018]and theCouncil of Scientific and Industrial Research, India [09/ 028(0920) 2014-EMR 1].
Corresponding Author: Kundu, P.P.; Department of Polymer Science and Technology, University of Calcutta, 92, A.P.C Road, India; email: ppk923@yahoo.com
Appears in Collections:Journal Publications [CH]

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