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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/2178
Title: A Mussel Mimetic, Bioadhesive, Antimicrobial Patch Based on Dopamine-Modified Bacterial Cellulose/rGO/Ag NPs: A Green Approach toward Wound-Healing Applications
Authors: Khamrai M.
Banerjee S.L.
Paul S.
Ghosh A.K.
Sarkar P.
Kundu, Patit Paban
Published in: ACS Sustainable Chemistry and Engineering
Abstract: A mussel mimetic transdermal patch was prepared using bacterial cellulose (BC), a green resource derived from Glucanoacetobacter xylinus (MTCC7795). To impart the mussel mimetic property, dopamine (DOPA), a catechol-containing compound, was used to modify the isolated BC via an amidation reaction between the carboxylated BC and DOPA, and the end product was successively characterized by 1H NMR and FTIR analysis. The free hydroxyl group of the DOPA moiety of DOPA-modified BC (BC-DOPA) was utilized to prepare BC-DOPA/rGO/Ag NPs, a composite film incorporating reduced graphene oxide/silver nanoparticles (rGO/Ag NPs). The antimicrobial action of the prepared film was determined against both Gram-positive (Staphylococcus aureus and Lysinibacillus fusiformis) and Gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacteria. The bactericidal property of the composite film was determined using the zone of inhibition (ZOI) method and live-dead assay (DAPI-PI analysis). The morphological transformation of bacteria upon the application of the composite film was observed through SEM analysis. The cell compatibility of the composite film over the NIH 3T3 fibroblast cell line was assessed through an XTT assay. The in vitro wound-healing assays over the NIH 3T3 cell line and A549 human lung epithelial cell line reveal that the presence of rGO and Ag NPs in the composite film accelerates the wound-healing process. © 2019 American Chemical Society.
Citation: ACS Sustainable Chemistry and Engineering (2019), 7(14): 12083-12097
URI: https://doi.org/10.1021/acssuschemeng.9b01163
http://repository.iitr.ac.in/handle/123456789/2178
Issue Date: 2019
Publisher: American Chemical Society
Keywords: Antimicrobial action
Bacterial cellulose
Mussel mimetic
Wound healing
ISSN: 21680485
Author Scopus IDs: 56641668300
56424674800
56464610400
56912675100
35461162400
35475516300
Author Affiliations: Khamrai, M., Advanced Polymer Laboratory, Department of Polymer Science and Technology, University of Calcutta, 92 Acharya Prafulla Chandra Road, Kolkata, 700009, India
Banerjee, S.L., Advanced Polymer Laboratory, Department of Polymer Science and Technology, University of Calcutta, 92 Acharya Prafulla Chandra Road, Kolkata, 700009, India
Paul, S., Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, 160012, India
Ghosh, A.K., Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, 160012, India
Sarkar, P., Advanced Polymer Laboratory, Department of Polymer Science and Technology, University of Calcutta, 92 Acharya Prafulla Chandra Road, Kolkata, 700009, India
Kundu, P.P., Advanced Polymer Laboratory, Department of Polymer Science and Technology, University of Calcutta, 92 Acharya Prafulla Chandra Road, Kolkata, 700009, India, Department of Chemical Engineering, Indian Institute of Technology (IIT), Roorkee, Uttarakhand, 247667, India
Corresponding Author: Kundu, P.P.; Advanced Polymer Laboratory, Department of Polymer Science and Technology, University of Calcutta, 92 Acharya Prafulla Chandra Road, India; email: ppk923@yahoo.com
Appears in Collections:Journal Publications [CH]

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