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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/21372
Title: Effect of earthworms in reduction and fate of antibiotic resistant bacteria (ARB) and antibiotic resistant genes (ARGs) during clinical laboratory wastewater treatment by vermifiltration
Authors: Arora S.
Saraswat S.
Rajpal A.
Shringi H.
Mishra R.
Sethi J.
Rajvanshi J.
Nag A.
Saxena S.
Kazmi, Absar Ahmad
Published in: Science of the Total Environment
Abstract: In the recent decades, the role of wastewater treatment plants has been entrenched for the dissemination of antibiotic resistant bacteria into the environment. The present study explores the dynamics of earthworms-microorganisms interactions involved in the high treatment efficacy of vermifiltration technology along with reduction of antibiotic resistant bacteria (ARB). This study is the first of its kind to investigate the performance efficacy of vermifilter (VF) for clinical laboratory wastewater treatment. The results of the study showed that earthworms and VF associated microbial community had a significant effect on Biochemical Oxygen Demand (BOD) and Chemical Oxygen Demand (COD) reduction (78–85%), coliforms and pathogen removal (>99.9%) and caused a significant shift in the prevalence pattern of ARB. Molecular profiling of resistance causing genes such as ESBL (blaSHV, blaTEM and blaCTX-M), MRSA (mec-A) and Colistin (mcr-1) confirmed the probable mechanisms behind the resistance pattern. The microbial community diversity in the influent, earthworm's coelomic fluid and gut and filter media layers associated with the VF assists in the formation of biofilm, which helps in the removal of pathogens from the wastewater. This biofilm formation further results in a paradigm shift in the resistance profile of ARB and ARG, specifically most effective against drugs, targeting cell wall and protein synthesis inhibition such as Ampicillin, Ticarcillin, Gentamicin and Chloramphenicol. These findings further validate vermifiltration technology as a sustainable and natural treatment technology for clinical laboratory wastewater, specifically for the removal of pathogens and antibiotic resistance. © 2021 Elsevier B.V.
Citation: Science of the Total Environment, 773
URI: https://doi.org/10.1016/j.scitotenv.2021.145152
http://repository.iitr.ac.in/handle/123456789/21372
Issue Date: 2021
Publisher: Elsevier B.V.
Keywords: Antibiotic resistance genes
Antimicrobial resistance
Clinical laboratory wastewater
Earthworms
Pathogens
Vermifilter
Wastewater treatment
ISSN: 489697
Author Scopus IDs: 14033888900
57220180492
55105210000
57221387230
57220182965
57220187162
57220177882
57220179929
57220187582
6701514485
Author Affiliations: Arora, S., Dr. B. Lal Institute of Biotechnology, Malviya Industrial Area, Malviya Nagar, Jaipur, 302017, India
Saraswat, S., Institute of Environmental and Occupational Health Sciences, School of Medicine, National Yang Ming University, Taiwan
Rajpal, A., Environmental Engineering Group, Department of Civil Engineering, Indian Institute of Technology Roorkee, India
Shringi, H., Dr. B. Lal Institute of Biotechnology, Malviya Industrial Area, Malviya Nagar, Jaipur, 302017, India
Mishra, R., Dr. B. Lal Institute of Biotechnology, Malviya Industrial Area, Malviya Nagar, Jaipur, 302017, India
Sethi, J., Dr. B. Lal Institute of Biotechnology, Malviya Industrial Area, Malviya Nagar, Jaipur, 302017, India
Rajvanshi, J., Dr. B. Lal Institute of Biotechnology, Malviya Industrial Area, Malviya Nagar, Jaipur, 302017, India
Nag, A., Dr. B. Lal Institute of Biotechnology, Malviya Industrial Area, Malviya Nagar, Jaipur, 302017, India
Saxena, S., Dr. B. Lal Institute of Biotechnology, Malviya Industrial Area, Malviya Nagar, Jaipur, 302017, India
Kazmi, A.A., Environmental Engineering Group, Department of Civil Engineering, Indian Institute of Technology Roorkee, India
Funding Details: The present research study is partially funded by the Department of Science & Technology-GoI (Grant No. DST/WTI/2K16/193) for financial support to this research and by internal mural grants (IMG) from Institutional Research Scientific Committee of the institute Dr. B. Lal Institute of Biotechnology, Jaipur. Department of Science and Technology, Ministry of Science and Technology, India, डीएसटी: DST/WTI/2K16/193
Corresponding Author: Arora, S.; Dr. B. Lal Institute of Biotechnology, 6E, Malviya Industrial Area, Malviya Nagar, India; email: sudiptiarora@gmail.com
Appears in Collections:Journal Publications [CE]

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