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Title: Strong larvicidal potential of Artemisia annua leaf extract against malaria (Anopheles stephensi Liston) and dengue (Aedes aegypti L.) vectors and bioassay-driven isolation of the marker compounds
Authors: Sharma G.
Kapoor H.
Chopra M.
Kumar K.
Agrawal V.
Published in: Parasitology Research
Abstract: Malaria and dengue are the two most important vector-borne human diseases caused by mosquito vectors Anopheles stephensi and Aedes aegypti, respectively. Of the various strategies adopted for eliminating these diseases, controlling of vectors through herbs has been reckoned as one of the important measures for preventing their resurgence. Artemisia annua leaf chloroform extract when tried against larvae of A. stephensi and A. aegypti has shown a strong larvicidal activity against both of these vectors, their respective LC50 and LC90 values being 0.84 and 4.91 ppm for A. stephensi and 0.67 and 5.84 ppm for A. aegypti. The crude extract when separated through column chromatography using petroleum ether-ethyl acetate gradient (0-100 %) yielded 76 fractions which were pooled into three different active fractions A, B and C on the basis of same or nearly similar R f values. The aforesaid pooled fractions when assayed against the larvae of A. stephensi too reported a strong larvicidal activity. The respective marker compound purified from the individual fractions A, B and C, were Artemisinin, Arteannuin B and Artemisinic acid, as confirmed and characterized through FT-IR and NMR. This is our first report of strong mortality of A. annua leaf chloroform extract against vectors of two deadly diseases. This technology can be scaled up for commercial exploitation. © 2013 Springer-Verlag Berlin Heidelberg.
Citation: Parasitology Research(2014), 113(1): 197-209
Issue Date: 2014
ISSN: 9320113
Author Scopus IDs: 55574123196
Author Affiliations: Sharma, G., Department of Botany, University of Delhi, Delhi 110007, India
Kapoor, H., Department of Botany, University of Delhi, Delhi 110007, India
Chopra, M., Dr. B.R. Ambedkar Centre for Biomedical Research, University of Delhi, Delhi 110007, India
Kumar, K., National Centre for Disease Control, Delhi 110054, India
Agrawal, V., Department of Botany, University of Delhi, Delhi 110007, India
Funding Details: Acknowledgements Authors are grateful to the Indian Council of Medical Research (ICMR), New Delhi for providing financial assistance in the form of major research project and in the form of SRF to GS. HK is indebted to University Grants Commission for the award of JRF.
Corresponding Author: Agrawal, V.; Department of Botany, University of Delhi, Delhi 110007, India; email:
Appears in Collections:Journal Publications [CH]

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