http://repository.iitr.ac.in/handle/123456789/2047| Title: | Anti-diabetic potential of alkaloid rich fraction from Capparis decidua on diabetic mice |
| Authors: | Sharma B. Salunke R. Balomajumder, Chandrajit Daniel S. Roy, Partha |
| Published in: | Journal of Ethnopharmacology |
| Abstract: | Ethno pharmacological relevance: Capparis decidua (CD) is a xerophytic shrub, found widely in the western parts of India, Pakistan and some of the Asian countries. The dried fruits are used as an ingredient in anti-diabetic compositions. Aim of the study: The present study was carried out to test the effect of alkaloid rich (AR) fraction from this plant in the management of diabetes. Materials and methods: Streptozotocin-induced diabetic mice were treated with the AR fraction for 28 days. On completion of the treatment, a range of parameters were tested including oral glucose tolerance test (OGTT), blood lipid profile, expression patterns of various glucose homeostatic enzyme genes and their activities. Results: Treatment of diabetic mice with AR fraction for 28 days significantly inhibited the acute elevation of blood glucose level during OGTT and also reduced total cholesterol (TC) and triglyceride (TG) content (p < 0.05). Activity of glucose-6-phosphatase (G6Pase) was attenuated by 44%, also liver and muscle glycogen content showed significant improvement (p < 0.05). The expression of different target genes like G6Pase, phosphoenolpyruvate carboxykinase (PEPCK), aldose reductase and tumor necrosis factor-? (TNF-?) showed significant reduction whereas glucose transporter-4 (Glut-4), peroxisome proliferator activated receptor-? (PPAR-?) and glucokinase (GK) improved remarkably. Conclusions: AR fraction showed promising results in terms of anti-diabetic activities establishing its candidacy for further purification and characterization of the individual alkaloids, in order to understand their mechanism of action. © 2009 Elsevier Ireland Ltd. All rights reserved. |
| Citation: | Journal of Ethnopharmacology(2010), 127(2): 457-462 |
| URI: | https://doi.org/10.1016/j.jep.2009.10.013 http://repository.iitr.ac.in/handle/123456789/2047 |
| Issue Date: | 2010 |
| Keywords: | Anti-diabetic Capparis decidua Hypoglycemic effect PPAR-? Serum lipids Streptozotocin-induced diabetic mice |
| ISSN: | 3788741 |
| Author Scopus IDs: | 56133941800 23991735800 35615087500 36522540500 35509207200 |
| Author Affiliations: | Sharma, B., Molecular Endocrinology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247 667, Uttarakhand, India Salunke, R., Molecular Endocrinology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247 667, Uttarakhand, India Balomajumder, C., Department of Chemical Engineering, Indian Institute of Technology Roorkee, Roorkee 247 667, Uttarakhand, India Daniel, S., Molecular Endocrinology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247 667, Uttarakhand, India Roy, P., Molecular Endocrinology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247 667, Uttarakhand, India |
| Funding Details: | Authors are thankful to Department of Science and Technology, Government of India (No. SR/FT/L-57/2004), University Grant Commission, Government of India, All India Council for Technical Education, Government of India for providing research support to P.R., B.S., and R.S., respectively. The authors would also like to thank Dr. A. Bandyopadhyay of Indian Institute of Chemical Biology, Kolkata, India, for kindly providing the antibodies used in this study. |
| Corresponding Author: | Roy, P.; Molecular Endocrinology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247 667, Uttarakhand, India; email: paroyfbs@iitr.ernet.in |
| Appears in Collections: | Journal Publications [BT] Journal Publications [CH] |
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