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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/20403
Title: AgNPs Ornamented Modified Bacterial Cellulose Based Self-Healable L-B-L Assembly via a Schiff Base Reaction: A Potential Wound Healing Patch
Authors: Khamrai M.
Banerjee S.L.
Paul S.
Ghosh A.K.
Sarkar P.
Kundu, Patit Paban
Published in: ACS Applied Bio Materials
Abstract: A layer-by-layer (L-B-L) bacterial cellulose (BC)-based transdermal patch has been prepared via a Schiff base reaction. The L-B-L assembly consisting of covalently cross-linked ethylene diamine-modified carboxymethylated BC isolated from the Glucanoacetobacter xylinus (MTCC7795) bacterial strain and aldehyde-modified pectin formed via a Schiff base reaction. The presence of the imine bond assists the self-healing process after being scratched in the presence of a pH 7.4 buffer solution monitored via optical microscopy, atomic force microscopy, and tensile strength analyses. The formation of the L-B-L assembly was confirmed using field-emission scanning electron microscopy (FESEM) analysis. Simultaneously, water swelling and deswelling studies were carried out to test its water retention efficiency. The presence of silver nanoparticles (AgNPs) has been confirmed by ultraviolet-visible spectroscopy and FESEM analyses. The antimicrobial activity of the AgNPs-incorporated transdermal patch has been examined over Staphylococcus aureus and Escherichia coli using the zone of inhibition method. Additionally, the cell viability assay was performed using the fluorescent dyes 4′,6-diamidino-2-phenylindole and propidium iodide. The AgNPs in the L-B-L assembly showed antimicrobial property against both types of bacteria. The cytotoxicity and wound healing property of the patch system have been studied over NIH 3T3 fibroblast and A549 epithelial cell lines. The L-B-L film also influenced the wound healing process of these two cell lines. ©
Citation: ACS Applied Bio Materials, 4(1): 428-440
URI: https://doi.org/10.1021/acsabm.0c00915
http://repository.iitr.ac.in/handle/123456789/20403
Issue Date: 2021
Publisher: American Chemical Society
Keywords: antimicrobial
bacterial cellulose
self-healing
transdermal patch
wound healing
ISSN: 25766422
Author Scopus IDs: 56641668300
56424674800
56464610400
56912675100
57211425713
35475516300
Author Affiliations: Khamrai, M., Advanced Polymer Laboratory, Department of Polymer Science and Technology, University of Calcutta, 92 A. P. C. Road, Kolkata, 700009, India
Banerjee, S.L., Advanced Polymer Laboratory, Department of Polymer Science and Technology, University of Calcutta, 92 A. P. C. Road, Kolkata, 700009, India
Paul, S., Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, 160012, India
Ghosh, A.K., Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, 160012, India
Sarkar, P., Advanced Polymer Laboratory, Department of Polymer Science and Technology, University of Calcutta, 92 A. P. C. Road, Kolkata, 700009, India
Kundu, P.P., Advanced Polymer Laboratory, Department of Polymer Science and Technology, University of Calcutta, 92 A. P. C. Road, Kolkata, 700009, India, Department of Chemical Engineering, Indian Institute of Technology (IIT) Roorkee, Roorkee, Uttarakhand 247667, India
Corresponding Author: Kundu, P.P.; Advanced Polymer Laboratory, 92 A. P. C. Road, India; email: ppk9233@yahoo.com
Appears in Collections:Journal Publications [CH]

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