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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/20159
Title: A chemical genetic approach using genetically encoded reporters to detect and assess the toxicity of plant secondary metabolites against bacterial pathogens
Authors: Dhyani R.
Srivastava S.K.
Shankar K.
Ghosh T.
Beniwal A.
Navani, Naveen Kumar
Published in: Journal of Hazardous Materials
Abstract: Plant secondary metabolites are emerging as attractive alternatives in the development of therapeutics against infectious and chronic diseases. Due to the present pandemic, therapeutics showing toxicity against bacterial pathogens and viruses are gaining interest. Plant metabolites of terpenoid and phenylpropanoid categories have known antibacterial and antiviral properties. These metabolites have also been associated with toxicity to eukaryotic cells in terms of carcinogenicity, hepatotoxicity, and neurotoxicity. Sensing methods that can report the exact antibacterial dosage, formation, and accumulation of these antibacterial compounds are needed. The whole-cell reporters for such antibacterial metabolites are cost-effective and easy to maintain. In the present study, battery of toxicity sensors containing fluorescent transcriptional bioreporters was constructed, followed by fine-tuning the response using gene-debilitated E. coli mutants. This study shows that by combining regulatory switches with chemical genetics strategy, it may be possible to detect and elucidate the mode of action of effective antibacterial plant secondary metabolites - thymol, cinnamaldehyde, eugenol, and carvacrol in both pure and complex formats. Apart from the detection of adulteration of pure compounds present in complex mixture of essential oils, this approach will be useful to detect authenticity of essential oils and thus reduce unintended harmful effects on human and animal health. © 2021 Elsevier B.V.
Citation: Journal of Hazardous Materials, 418
URI: https://doi.org/10.1016/j.jhazmat.2021.126399
http://repository.iitr.ac.in/handle/123456789/20159
Issue Date: 2021
Publisher: Elsevier B.V.
Keywords: Adulteration
Bioreporters
Complex mixture
Plant secondary metabolites
ISSN: 3043894
Author Scopus IDs: 57212492419
57202143131
57223098652
57197638847
57188871232
6507073924
Author Affiliations: Dhyani, R., Department of Biotechnology, Indian Institute of Technology RoorkeeUttarakhand 247667, India
Srivastava, S.K., Department of Biotechnology, Indian Institute of Technology RoorkeeUttarakhand 247667, India
Shankar, K., Department of Biotechnology, Indian Institute of Technology RoorkeeUttarakhand 247667, India
Ghosh, T., Department of Biotechnology, Indian Institute of Technology RoorkeeUttarakhand 247667, India
Beniwal, A., Department of Biotechnology, Indian Institute of Technology RoorkeeUttarakhand 247667, India
Navani, N.K., Department of Biotechnology, Indian Institute of Technology RoorkeeUttarakhand 247667, India
Funding Details: This research was supported by National Agricultural Science Fund (NASF) Grant no. NASF/Minimization-6024/2017-18 of the Indian Council of Agricultural Research (ICAR) funded by the Government of India . We thank Professor Gerry Wright at McMaster University, Hamilton, Ontario, Canada for the gift of E.coli strain ΔbamBΔtolC. We thank Professor Steven Lindow at the University of California, Berkeley USA for the gift of plasmid pPROBE-TT′-GFP. We also thank Professor Ranjana Pathania of the Indian Institute of Technology, Roorkee for critically reading the manuscript. NASF/Minimization-6024/2017-18; University of California Berkeley, UCB; Indian Council of Agricultural Research, ICAR
Corresponding Author: Navani, N.K.; Department of Biotechnology, India; email: naveen.navani@bt.iitr.ac.in
Appears in Collections:Journal Publications [BT]

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