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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/20149
Title: Dissecting the anti-biofilm potency of kappa-carrageenan capped silver nanoparticles against Candida species
Authors: Gupta P.
Goel A.
Singh K.R.
Meher M.K.
Gulati K.
Poluri, Krishna Mohan
Published in: International Journal of Biological Macromolecules
Abstract: Global antimicrobial crisis and advent of drug resistant fungal strains has substantially distressed disease management for clinicians. Biodegradable silver nanoparticles (AgNps) emerge as an excellent alternative remedial option. In the current study, the anti-biofilm activity of microwave irradiated kappa-carrageenan (CRG) capped AgNps against Candida albicans, and Candida glabrata was investigated in terms of their effect on reactive oxygen species (ROS) generation, cellular morphology, biochemical composition, and the activity of enzymes of extracellular matrix. Minimum inhibitory concentration and fungicidal concentration value of CRG-AgNps against both Candida spp. ranged between 400 and 500 μg/mL. The 80% of Candida biofilm was inhibited and eradicated by CRG-AgNps at a concentration of ~300 μg/mL. Microscopic studies indicate that CRG-AgNps caused morphological damage through membrane disruption and pore formation. Further, CRG-AgNps generated ROS in a concentration-dependent manner and modulated the composition of Candida biofilm ECM by increasing the carbohydrate and eDNA content. CRG-AgNps also significantly inactivated the hydrolytic enzymes, thus hindering the biofilm forming ability. In conclusion, all these results suggest that the CRG-AgNps are potential antifungal agents against Candida biofilms, and they inhibit/eradicate the fungal biofilms through multiple signalling mechanisms. © 2021 Elsevier B.V.
Citation: International Journal of Biological Macromolecules, 172: 30-40
URI: https://doi.org/10.1016/j.ijbiomac.2021.01.035
http://repository.iitr.ac.in/handle/123456789/20149
Issue Date: 2021
Publisher: Elsevier B.V.
Keywords: Antimicrobial agents
Candida
Fungal biofilm
Kappa carrageenan
Reactive oxygen species
Silver nanocomposites
ISSN: 1418130
Author Scopus IDs: 56658955600
57204919554
57221031141
57195152503
56998446900
55842079400
Author Affiliations: Gupta, P., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667, India
Goel, A., Centre for Nanotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667, India
Singh, K.R., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667, India
Meher, M.K., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667, India
Gulati, K., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667, India
Poluri, K.M., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667, India, Centre for Nanotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667, India
Funding Details: PG acknowledges the financial support of DBT-RAship, from the Department of Biotechnology (DBT), Government of India. KRS acknowledges the receipt of DBT-JRF/SRF from DBT, India. KMP acknowledge the receipt of Grant CRG/2018/001329 and SB/YS/LS-380/2013 from SERB-DST, and DBT-IYBA fellowship ? BT/07/IYBA/2013-19 from the Government of India. The authors are thankful to the FESEM facility at Institute Instrumentation Centre, IIT Roorkee. BT/07/IYBA/2013-19; Department of Biotechnology, Ministry of Science and Technology, India, DBT: CRG/2018/001329, SB/YS/LS-380/2013; Indian Institute of Technology Roorkee, IITR
Corresponding Author: Poluri, K.M.; Department of Biotechnology, India; email: krishna.poluri@bt.iitr.ac.in
Appears in Collections:Journal Publications [BT]

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