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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/20117
Title: One-Step Fabrication of Low-Cost, Autoclavable, and Multifunctional Silk-Based Nanofibrous Permeable Hanging Cell Culture Inserts for Various Biological Applications
Authors: Basak S.
Kumar V.
Gopinath, Packirisamy
Published in: ACS Omega
Abstract: Hanging cell culture inserts are most widely used in vitro cell culture devices, which provide a freestanding multichamber setup for various co-culture and triculture systems. Apart from being costly, the commercial inserts do not provide enough choices regarding polymer types and pore sizes. Most importantly, commercially available inserts are two-dimensional multiporous membrane-based devices. Herein, we report a one-step fabrication process of the multifunctional nanofiber-based permeable hanging cell culture insert using electrospinning. These fabricated nanofibrous membranes' attached inserts have advantages such as low cost, ready availability, easy fabrication, tunable porosity, autoclavability, and biomaterial-based nanofibrous membranes. The inserts without nanofibrous membrane can also be reused by autoclaving them and electrospun nanofibrous membrane on it according to the application. We have also confirmed its suitability for extensive use in the field of in vitro cell culture by analyzing its adherence and toxicity results on breast cancer cell line (MCF-7). These hanging cell culture inserts are thus a potent product for various cell culture assays such as cell migration for wound healing, cancer metastasis, and other tissue engineering applications. © 2021 The Authors. Published by American Chemical Society.
Citation: ACS Omega, 6(11): 7605-7614
URI: https://doi.org/10.1021/acsomega.0c06135
http://repository.iitr.ac.in/handle/123456789/20117
Issue Date: 2021
Publisher: American Chemical Society
ISSN: 24701343
Author Scopus IDs: 57219773998
57208634573
56242963200
Author Affiliations: Basak, S., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, 247667, India
Kumar, V., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, 247667, India
Packirisamy, G., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, 247667, India, Nanobiotechnology Laboratory, Centre for Nanotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, 247667, India
Funding Details: S.B. is thankful to the Department of Biotechnology (DBT), Government of India, for providing the fellowship. V.K. is thankful to the Ministry of Education (MoE), Government of India, for the fellowship and BIRAC-SRISTI GYTI Award (2019). The authors acknowledge the Department of Biotechnology, Government of India (BT/PR 25095/NER/95/1011/2017), and Shastri Institutional Collaborative Research Grant (SICRG) 2020–21 for the financial support. Centre for Nanotechnology, Department of Chemistry and Institute Instrumentation Centre, Indian Institute of Technology Roorkee, is sincerely acknowledged for providing various analytical facilities. Department of Biotechnology, Ministry of Science and Technology, India, DBT: BT/PR 25095/NER/95/1011/2017; Kementerian Pendidikan Malaysia, KPM
Corresponding Author: Packirisamy, G.; Department of Biotechnology, India; email: gopi@bt.iitr.ac.in
Appears in Collections:Journal Publications [BT]

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