Biodegradation of phenol and cyanide by Pseudomonas putida MTCC 1194: an experimental and modeling study

Abstract

In this study, the biodegradation of phenol and cyanide from aqueous solution by Pseudomonas putida MTCC 1194 was carried out. The effect of initial concentration of phenol and cyanide on the microbial growth was estimated in the range of initial concentration 100–1,500 mg/L of phenol and 10–150 mg/L of cyanide. A maximum experimental specific growth rate was observed 0.0958 h?1 at 250 mg/L of phenol and 0.1590 h?1 at 25 mg/L of cyanide concentration in the growth medium. Specific growth rate was observed to follow substrate inhibition kinetics. Non-linear regression analysis technique with error function Marquardt’s percent standard deviation was used to predict the model parameters. Seven growth kinetic models for the mono substrate system were calculated and corresponding interaction parameters were predicted. The experimental data was found well fitted with Haldane, Edward, Yano and Koga, Luong and Han and Levenspiel models as compared to Monod and Powell model. For binary substrate system, sum kinetics with interaction parameters model was used. Three-half-order degradation kinetic model was found to be fit, well with experimental data. This study presented that P. putida MTCC 1194 was very effective for the removal of phenol and cyanide from aqueous solutions. © 2016 Balaban Desalination Publications. All rights reserved.