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Title: Alkali pretreatment of wheat straw followed by microbial hydrolysis for bioethanol production
Authors: Tsegaye B.
Balomajumder, Chandrajit
Roy, Partha
Published in: Environmental Technology (United Kingdom)
Abstract: The combination of NaOH pretreatment and microorganisms isolated from termite was used for releasing wrapped polysaccharides from wheat straw biomass matrix. Different concentrations of NaOH (1%, 3%, 5%, 7% and 10%) were considered to remove lignin and to release polysaccharides as a pretreatment method at 80°C for 4 h before subjecting it to microbial hydrolysis. Data obtained from compositional analysis of pretreated wheat straws show that a significant amount of cellulose and lignin were released after NaOH pretreatments. The amount of cellulose and lignin released was increased with increasing concentration of NaOH in the pretreatment solution. Further analysis of X-Ray diffraction, field emission scanning electron microscope and Fourier transform infrared spectroscopy confirms the removal of lignin and release of cellulose. About 69.5% of lignin was solubilized and 72.67% of cellulose was released after 10% NaOH pretreatment which was the maximum. Data from spectrophotometric analysis of reducing sugar by the 3,5-dinitrosalycilic acid method show that 83.68% (0.706 g/100 ml) of polysaccharides were converted to glucose and xylose by isolated bacteria after the 15th day of hydrolysis. © 2017, © 2017 Informa UK Limited, trading as Taylor & Francis Group.
Citation: Environmental Technology (United Kingdom)(2019), 40(9): 1203-1211
Issue Date: 2019
Publisher: Taylor and Francis Ltd.
Keywords: cellulose
Microbial hydrolysis
NaOH pretreatment
wheat straw
ISSN: 9593330
Author Scopus IDs: 57200083531
Author Affiliations: Tsegaye, B., Department of Chemical Engineering, Indian Institute of Technology Roorkee, Roorkee, India
Balomajumder, C., Department of Chemical Engineering, Indian Institute of Technology Roorkee, Roorkee, India
Roy, P., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, India
Funding Details: One of the authors, Bahiru Tsegaye would like to thank the Ministry of Education of Ethiopia for providing fellowship.
Corresponding Author: Tsegaye, B.; Department of Chemical Engineering, Indian Institute of Technology RoorkeeIndia; email:
Appears in Collections:Journal Publications [BT]
Journal Publications [CH]

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