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Title: Mass Transfer and Rheological Characteristics in a Stirred Tank Bioreactor for Cultivation of Escherichia coli BL21
Authors: Lone S.R.
Kumar, Vimal
Seay J.R.
Englert D.L.
Hwang H.T.
Korean Society for Biotechnology and Bioengineering
Published in: Biotechnology and Bioprocess Engineering
Abstract: With the rapid growth of pharmaceutical and biotechnology industry, stirred tank bioreactors have received much attention due to simple design, easy control of operating conditions, and low operating cost. In the development of commercial processes, however, a transition from laboratory to industrial scale faces great challenges because many properties related to size change nonlinearly as a system increases. In this context, along with an understanding of fluid dynamics, application of an efficient method for scale-up is critical for designing successful industrial process. Particularly in cell cultivation processes, it is important to evaluate the oxygen transfer and viscous properties of liquid medium. In the present study, the effect of various key operating variables such as agitation rate and aeration rate, impeller diameter, and bioreactor working volume for different impellers on the volumetric mass transfer coefficient (k a) have been investigated in a stirred tank bioreactor for cultivating Escherichia coli BL21. It was found that the k a tends to increase with the operating variables except the bioreactor working volume. Among the tested impellers, the pitched blade was observed to be most promising because of relatively higher k a but less shear force owing to its low power number. It was also found that the liquid medium with E. coli behaves as a Newtonian liquid. Compared to conventional designs of Rushton turbines, dislocated Rushton turbine was found to deliver higher k a. Finally, using dimensional analysis, the k a for different impeller configurations was correlated in the form of dimensionless groups, suggesting that this approach can be used for predicting k a in different scales of stirred tank bioreactors.
Citation: Biotechnology and Bioprocess Engineering(2020), 25(5): 766-776
Issue Date: 2020
Publisher: Korean Society for Biotechnology and Bioengineering
Keywords: dimensional analysis
Escherichia coli
gas-liquid system
mass transfer coefficient
stirred tank bioreactor
ISSN: 12268372
Author Scopus IDs: 57200274227
Author Affiliations: Lone, S.R., Department of Chemical Engineering, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, 247667, India, Department of Chemical & Materials Engineering, University of Kentucky, 4810 Alben Barkley Drive, Paducah, KY 42002, United State
Funding Details: .Indian Institute of Technology Roorkee, IITR Ministry of Coal, Government of India University of Kentucky, UK Division of Human Resource Development, HRD.This work was jointly supported by the collaborative research program of the University of Kentucky and the Indian Institute of Technology Roorkee. The financial assistance by the Ministry of Human Resource Development (MHRD), Government of India is also
Corresponding Author: Hwang, H.T.; Department of Chemical & Materials Engineering, 4810 Alben Barkley Drive, United States; email:
Appears in Collections:Journal Publications [CH]

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