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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/1867
Title: Valorization of glycerol into polyhydroxyalkanoates by sludge isolated bacillus sp. RER002: Experimental and modeling studies
Authors: Zafar M.
Kumar S.
Kumar S.
Agrawal J.
Dhiman, Amit KumarK.
Published in: Chemical Product and Process Modeling
Abstract: In this study, the feasibility of glycerol valorization into homo- and hetero-polymers of polyhydroxyalkanoates by a sludge isolated Bacillus sp. RER002 in a 3 L bioreactor was investigated. A mathematical model including logistic, Luedeking-Piret, and Luedeking-Piret-like equations that simulated the active residual biomass growth, P(3HB) synthesis, and glycerol consumption, respectively, was developed. In order to describe the dynamics of batch P(3HB) production, the model kinetic parameters viz., ?max, K1, K2, ?, ?, and KN were optimized using the stochastic search-based genetic algorithm. The synthesis of P(3HB) wasobserved to be highly growth associated and partially non-growth associated as reflected in a significant higher values of K1 (0.2435-0.5477) than K2 (2.2 × 10-6 to 9.1 × 10-3) within the glycerol concentration range of 10-40 g/L. Besides, the maximum 3.2g/L of copolymer [P(3HAscl-co-3HAmcl)] was observed at 30 g/L of glycerol concentration in synthetic crude glycerol medium with a yield coefficient (YP/S) of 0.16 g/g. Furthermore, the analyses of chemical and thermal properties of copolymer P(3HAscl-co-3HAmcl) revealed its enhanced material properties which make it suitable for various applications. © by De Gruyter 2014.
Citation: Chemical Product and Process Modeling (2014), 9(2): 117-131
URI: https://doi.org/10.1515/cppm-2014-0011
http://repository.iitr.ac.in/handle/123456789/1867
Issue Date: 2014
Publisher: Walter de Gruyter GmbH
Keywords: genetic algorithm
glycerol
modeling
P(HAscl-co-HAmcl)
valorization
ISSN: 21946159
Author Scopus IDs: 57197240378
57209548014
55367444400
56435703200
8548369300
Author Affiliations: Zafar, M., Department of Chemical Engineering, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, 247 667, India, Department of Biological Engineering, Shobhit University, Meerut, Uttar Pradesh, 250 110, India
Kumar, S., Department of Chemical Engineering, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, 247 667, India
Kumar, S., Department of Chemical Engineering, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, 247 667, India
Agrawal, J., Department of Chemical Engineering, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, 247 667, India
Dhiman, A.K., Department of Chemical Engineering, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, 247 667, India
Funding Details: Acknowledgment: This study is financially supported by Ministry of Human Resources and Development, Govt. of India, New Delhi.
Corresponding Author: Kumar, S.; Department of Chemical Engineering, Indian Institute of Technology RoorkeeIndia
Appears in Collections:Journal Publications [CH]

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