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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/1790
Title: Use of response surface methodology for optimizing process parameters for the production of ?-amylase by Aspergillus oryzae
Authors: Francis F.
Sabu A.
Nampoothiri K.M.
Ramachandran S.
Ghosh, Sanjoy
Szakacs G.
Pandey A.
Published in: Biochemical Engineering Journal
Abstract: Optimization of three parameters (incubation temperature, initial substrate moisture and inoculum size) was attempted by using a Box-Behnken design under the response surface methodology for the optimal production of ?-amylase by Aspergillus oryzae NRRL 6270 in solid-state fermentation (SSF). Spent brewing grains (SBG) was used as sole carbon source. The experimental data was fitted into a polynomial model for the yield of enzyme and an optimum level was arrived at which nutrient supplements were optimized. A Plackett-Burman design was employed to screen nineteen nutrient components for their influence on ?-amylase production by the fungal culture. Three components (soybean meal, calcium chloride and magnesium sulphate) were selected based on their positive influence on enzyme formation. A Box-Behnken design was employed to optimize their composition, which showed that an incubation temperature of 30 °C, an initial moisture of 70% and an inoculum rate of 1 × 107 spores/g dry substrate were the best conditions to produce ?-amylase with A. oryzae NRRL 6270 on SBG. Under optimized conditions of SSF, about 20% increase in enzyme yield was observed. © 2002 Elsevier Science B.V. All rights reserved.
Citation: Biochemical Engineering Journal (2003), 15(2): 107-115
URI: https://doi.org/10.1016/S1369-703X(02)00192-4
http://repository.iitr.ac.in/handle/123456789/1790
Issue Date: 2003
Publisher: Elsevier
Keywords: Aspergillus oryzae
Response surface methodology
Solid-state fermentation
Spent brewing grains
?-Amylase
ISSN: 1369703X
Author Scopus IDs: 7004815612
6602534207
6602697956
7203055774
56603000600
35567549000
7201771319
Author Affiliations: Francis, F., Biotechnology Division, Regional Research Laboratory, CSIR, Trivandrum, Kerala 695019, India, Centre for Biotechnology, Anna University, Chennai 600025, India
Sabu, A., Biotechnology Division, Regional Research Laboratory, CSIR, Trivandrum, Kerala 695019, India
Nampoothiri, K.M., Biotechnology Division, Regional Research Laboratory, CSIR, Trivandrum, Kerala 695019, India
Ramachandran, S., Biotechnology Division, Regional Research Laboratory, CSIR, Trivandrum, Kerala 695019, India
Ghosh, S., Centre for Biotechnology, Anna University, Chennai 600025, India
Szakacs, G., Dept. of Agric. Chemical Technology, Budapest Univ. of Technol./Economics, 1111 Budapest, Gellerter 4, Hungary
Pandey, A., Biotechnology Division, Regional Research Laboratory, CSIR, Trivandrum, Kerala 695019, India
Corresponding Author: Pandey, A.; Biotechnology Division, Regional Research Laboratory, CSIR, Trivandrum, Kerala 695019, India; email: pandey@csrrltrd.ren.nic.in
Appears in Collections:Journal Publications [BT]

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