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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/1760
Title: Purification and characterization of a trypsin inhibitor from seeds of Murraya koenigii
Authors: Shee C.
Sharma A.
Published in: Journal of Enzyme Inhibition and Medicinal Chemistry
Abstract: A protein with trypsin inhibitory activity was purified to homogeneity from the seeds of Murraya koenigii (curry leaf tree) by ion exchange chromatography and gel filtration chromatography on HPLC. The molecular mass of the protein was determined to be 27 kDa by SDS-PAGE analysis under reducing conditions. The solubility studies at different pH conditions showed that it is completely soluble at and above pH 7.5 and slowly precipitates below this pH at a protein concentration of 1 mg/ml. The purified protein inhibited bovine pancreatic trypsin completely in a molar ratio of 1:1.1. Maximum inhibition was observed at pH 8.0. Kinetic studies showed that Murraya koenigii trypsin inhibitor is a competitive inhibitor with an equilibrium dissociation constant of 7 × 10-9 M. The N-terminal sequence of the first 15 amino acids showed no similarity with any of the known trypsin inhibitors, however, a short sequence search showed significant homology to a Kunitz-type chymotrypsin inhibitor from Erythrina variegata.
Citation: Journal of Enzyme Inhibition and Medicinal Chemistry(2007), 22(1): 115-120
URI: https://doi.org/10.1080/14756360601027332
http://repository.iitr.ac.in/handle/123456789/1760
Issue Date: 2007
Keywords: Murraya koenigii
Proteinase inhibitor
Solubility
Trypsin inhibitor
ISSN: 14756366
Author Scopus IDs: 16032104700
57214355701
Author Affiliations: Shee, C., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee-247 667, India
Sharma, A., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee-247 667, India
Funding Details: We thank Prof. T. P. Singh and Dr. Sujata Sharma for N-terminal sequencing and Dr. S. Karthikeyan for helpful discussion. Chandan Shee thanks the Ministry of Human Resource Development, Government of India for financial support.
Corresponding Author: Sharma, A.K.; Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee-247 667, India
Appears in Collections:Journal Publications [BT]

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