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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/1756
Title: Accumulation of p-hydroxybenzoic acid in hairy roots of Daucus carota
Authors: Sircar, Debabrata
Roychowdhury A.
Mitra A.
Published in: Journal of Plant Physiology
Abstract: We report here the accumulation of p-hydroxybenzoic acid in Agrobacterium rhizogenes-induced hairy root cultures of Daucus carota. This phenolic acid finds application in food, pharmaceutical and polymer industries. Metabolic profiling of phenolics by HPLC/ESI-MS from these hairy roots showed a considerable amount of p-hydroxybenzoic acid accumulation both in cytosol and in the cell wall. Analyses of HCl and NaOH treated soluble phenolic fractions resulted in the elution of peaks with same retention time and similar UV-absorption spectra as observed with p-hydroxybenzoic acid standard. This suggests that p-hydroxybenzoic acid is present in the cytosol as free-form (unconjugated). A correlation has been drawn between the accumulation of soluble and wall-bound phenolic acids on a time-course basis. An apparent absence of any p-hydroxybenzoic acid-glucoside supports this observation, which in turn encourages the idea of its incorporation in the cell wall in an alkaline-labile form. © 2006 Elsevier GmbH. All rights reserved.
Citation: Journal of Plant Physiology(2007), 164(10): 1358-1366
URI: https://doi.org/10.1016/j.jplph.2006.08.002
http://repository.iitr.ac.in/handle/123456789/1756
Issue Date: 2007
Publisher: Elsevier GmbH
Keywords: Daucus carota
Hairy roots
p-Hydroxybenzoic acid
Phenolic acids
ISSN: 1761617
Author Scopus IDs: 21743930700
14012247100
7402543434
Author Affiliations: Sircar, D., Natural Product Biotechnology Group, Agricultural and Food Engineering Department, Indian Institute of Technology Kharagpur, Kharagpur, 721 302, India
Roychowdhury, A., Natural Product Biotechnology Group, Agricultural and Food Engineering D
Funding Details: This work was supported by a research grant (no.SP/SR/BB-40/2002 to A. Mitra) from the Department of Science and Technology (DST), Government of India. D. Sircar acknowledges the Council of Scientific and Industrial Research (CSIR) for the award of an ind
Corresponding Author: Mitra, A.; Natural Product Biotechnology Group, Agricultural and Food Engineering Department, Indian Institute of Technology Kharagpur, Kharagpur, 721 302, India; email: adin@iitkgp.ac.in
Appears in Collections:Journal Publications [BT]

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