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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/1748
Title: Optimization of nitrilase production from a new thermophilic isolate
Authors: Khandelwal A.K.
Nigam V.K.
Choudhury, Bijan
Mohan M.K.
Ghosh P.
Published in: Journal of Chemical Technology and Biotechnology
Abstract: A new thermostable nitrilase-producing isolate identified as Streptomyces sp. MTCC 7546 has been studied extensively for the optimization of enzyme production operating in batch mode. The benzonitrile was observed as inducer of nitrilase production. The isolate showed maximum nitrilase production after 24h of incubation at optimal conditions. The strain grows well on a variety of carbon sources and produces the nitrilase that catalyses the hydrolysis of nitriles to acids without formation of amides. The enzyme is mostly active against mono- and di-aliphatic nitriles (10 mmol L-1) at pH of 7.4 and at a temperature of 50°C. © 2007 Society of Chemical Industry.
Citation: Journal of Chemical Technology and Biotechnology (2007), 82(7): 646-651
URI: https://doi.org/10.1002/jctb.1721
http://repository.iitr.ac.in/handle/123456789/1748
Issue Date: 2007
Keywords: Aliphatic nitriles
Biotransformation
Nitrilase
Streptomyces
Thermophilic
ISSN: 2682575
Author Scopus IDs: 18433918500
7004690752
7103333522
12242902600
57212696908
Author Affiliations: Khandelwal, A.K., Birla Institute of Scientific Research, Statue Circle, Jaipur-302 001 Rajasthan, India
Nigam, V.K., Birla Institute of Scientific Research, Statue Circle, Jaipur-302 001 Rajasthan, India
Choudhury, B., Indian Institute of Technology, Roorkee, Uttaranchal, India
Mohan, M.K., Birla Institute of Scientific Research, Statue Circle, Jaipur-302 001 Rajasthan, India
Ghosh, P., Birla Institute of Scientific Research, Statue Circle, Jaipur-302 001 Rajasthan, India
Corresponding Author: Nigam, V.K.; Birla Institute of Scientific Research, Statue Circle, Jaipur-302 001 Rajasthan, India; email: nigam_vinod@yahoo.com
Appears in Collections:Journal Publications [BT]

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