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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/1727
Title: Biodesulfurization of dibenzothiophene using recombinant Pseudomonas strain
Authors: Meesala L.
Balomajumder C.
Chatterjee S.
Roy P.
Published in: Journal of Chemical Technology and Biotechnology
Abstract: Background: The sulfur content in crude oil available from various sources ranges from 0.03 to values as high as 8.0 wt%. These high quantities of sulfur must be removed before the crude oil is processed because combustion of this oil would result in severe environmental pollution, such as acid rain. Due to high utility and operating costs, the conventional hydrodesulfurization process (HDS) is considered to be uneconomic. The biotechnological option, biodesulfurization (BDS) seems an attractive low cost, environmentally benign technology. Results: This paper reports the development of a recombinant strain of bacteria designed by introducing desulfurizing, dsz genes containing plasmid pSAD 225-32, which was isolated from Rhodococcus erythropolis IGTS8 into a gram negative solvent-tolerant bacterium, Pseudomonas putida (MTCC 1194). This recombinant bacterium can desulfurize the dibenzothiophene (DBT) in the sulfur selective 4S-pathway. It has been observed that for the same concentration of DBT, the recombinant strain's growth rate is greater than that of the parent strain. Increasing the concentration of DBT resulted in an increase of lag phase as well as decreased growth rate, which shows that the bacteria is following substrate inhibition type kinetics. This genetically modified bacterium can desulfurize 73.1% of 1.2 mmolL-1 DBT (dissolved in ethanol) in 67 h of cultivation time using growing cells. Conclusions: It is concluded that further research in this area of biodesulfurization using genetically modified organisms may remove the bottlenecks presently in the way of commercialization of the BDS process. © 2007 Society of Chemical Industry.
Citation: Journal of Chemical Technology and Biotechnology (2008), 83(3): 294-298
URI: https://doi.org/10.1002/jctb.1807
http://repository.iitr.ac.in/handle/123456789/1727
Issue Date: 2008
Keywords: Biodesulfurization
Dibenzothiophene
Gram-negative bacteria
Recombinant bacteria
ISSN: 2682575
Author Scopus IDs: 23768506200
35615087500
17341895000
35509207200
Author Affiliations: Meesala, L., Department of Chemical Engineering, Indian Institute of Technology, Roorkee 247667 Uttarakhand, India
Balomajumder, C., Department of Chemical Engineering, Indian Institute of Technology, Roorkee 247667 Uttarakhand, India
Chatterjee, S., Department of Biotechnology, Indian Institute of Technology, Roorkee 247667 Uttarakhand, India
Roy, P., Department of Biotechnology, Indian Institute of Technology, Roorkee 247667 Uttarakhand, India
Corresponding Author: Balomajumder, C.; Department of Chemical Engineering, Indian Institute of Technology, Roorkee 247667 Uttarakhand, India; email: cbmajumder@yahoo.com
Appears in Collections:Journal Publications [BT]

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