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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/1725
Title: Enhanced delignification of mixed wood pulp by Aspergillus fumigatus laccase mediator system
Authors: Vivekanand V.
Dwivedi P.
Sharma A.
Sabharwal N.
Singh, Rajesh Pratap
Published in: World Journal of Microbiology and Biotechnology
Abstract: An environmentally sound biobleaching to get high quality paper pulp from mixed wood pulp was attempted employing laccase from Aspergillus fumigatus VkJ2.4.5 for lignin removal. Laccase treatment was performed in the presence of a mediator N-hydroxybenzotriazole (HBT, 1.5% w/w), resulting into notably higher level of delignification of the pulp. Enzyme at 10 Ug-1 of pulp at 50°C, pH 6.0, for 2 h with a pulp consistency of 10% was found suitable for enabling maximum decrease in the kappa number. The kappa number and yellowness decreased by 14 and 4% whereas ISO brightness improved by 7%. The presence of a characteristic peak at 280 nm indicated the presence of lignin in the effluent during biobleaching. Analysis of FTIR spectra of residual lignin revealed characteristic modifications following enzymatic bleaching by laccase mediator system (LMS). Variations in morphology and crystallinity of pulp were evaluated by scanning electron microscopy and X-ray diffraction analysis. © 2008 Springer Science+Business Media B.V.
Citation: World Journal of Microbiology and Biotechnology(2008), 24(12): 2799-2804
URI: https://doi.org/10.1016/j.molstruc.2007.07.046
http://repository.iitr.ac.in/handle/123456789/1725
Issue Date: 2008
Keywords: Aspergillus fumigatus
Biobleaching
Laccase
Pulp
ISSN: 9593993
Author Scopus IDs: 57211114758
15044112000
55605770474
36793648600
57208931324
Author Affiliations: Vivekanand, V., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247 667, India
Dwivedi, P., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247 667, India
Sharma, A., Department of Biotechnolog
Funding Details: Acknowledgments National Doctoral Fellowship of All India Council for Technical Education, New Delhi, awarded to first author and a Senior Research Fellowship of Council of Scientific and Industrial Research, New Delhi, India awarded to second author are
Corresponding Author: Singh, R. P.; Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247 667, India; email: rpsbsfbs@iitr.ernet.in
Appears in Collections:Journal Publications [BT]

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