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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/1707
Title: Purification, crystallization and preliminary crystallographic studies of a kunitz-type proteinase inhibitor from tamarind (tamarindus indica) seeds
Authors: Patil D.N.
Preeti
Chaudhry A.
Sharma, Ashwani Kumar
Tomar, Shailly
Kumar, Pravindra R.Manish
Published in: Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Abstract: A Kunitz-type proteinase inhibitor has been purified from tamarind (Tamarindus indica) seeds. SDS-PAGE analysis of a purified sample showed a homogeneous band corresponding to a molecular weight of 21 kDa. The protein was identified as a Kunitz-type proteinase inhibitor based on N-terminal amino-acid sequence analysis. It was crystallized by the vapour-diffusion method using PEG 6000. The crystals belonged to the orthorhombic space group C2221, with unit-cell parameters a = 37.2, b = 77.1, c = 129.1 Å. Diffraction data were collected to a resolution of 2.7 Å. Preliminary crystallographic analysis indicated the presence of one proteinase inhibitor molecule in the asymmetric unit, with a solvent content of 44%. © 2009 International Union of Crystallography All rights reserved.
Citation: Acta Crystallographica Section F: Structural Biology and Crystallization Communications(2009), 65(7): 736-738
URI: https://doi.org/10.1107/S1744309109023495
http://repository.iitr.ac.in/handle/123456789/1707
Issue Date: 2009
Keywords: Kunitz-type proteinase inhibitors
Tamarindus indica
ISSN: 17443091
Author Scopus IDs: 26431600400
35103173800
35101476600
57214355701
57203506001
55064809000
Author Affiliations: Patil, D.N., Department of Biotechnology, Indian Institute of Technology, Roorkee 247667, India
Preeti, Department of Biotechnology, Indian Institute of Technology, Roorkee 247667, India
Chaudhry, A., Department of Biotechnology, Indian Institute of Technology, Roorkee 247667, India
Sharma, A.K., Department of Biotechnology, Indian Institute of Technology, Roorkee 247667, India
Tomar, S., Department of Biotechnology, Indian Institute of Technology, Roorkee 247667, India
Kumar, P., Department of Biotechnology, Indian Institute of Technology, Roorkee 247667, India
Corresponding Author: Tomar, S.; Department of Biotechnology, Indian Institute of Technology, Roorkee 247667, India; email: shailfbt@iitr.ernet.in
Appears in Collections:Journal Publications [BT]

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