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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/1706
Title: Crystallization and preliminary X-ray diffraction analysis of the complex of Kunitz-type tamarind trypsin inhibitor and porcine pancreatic trypsin
Authors: Tomar, S.
Patil D.N.
Datta M.
Tapas S.
Preeti
Chaudhary A.
Sharma, Ashwani Kumar
Tomar, Shailly
Kumar, Pravindra R.Manish
Published in: Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Abstract: The complex of Tamarindus indica Kunitz-type trypsin inhibitor and porcine trypsin has been crystallized by the sitting-drop vapour-diffusion method using ammonium acetate as precipitant and sodium acetate as buffer. The homogeneity of complex formation was checked by size-exclusion chromatography and further confirmed by reducing SDS-PAGE. The crystals diffracted to 2.0 Å resolution and belonged to the tetragonal space group P41, with unit-cell parameters a = b = 57.1, c = 120.1 Å. Preliminary X-ray diffraction analysis indicated the presence of one unit of inhibitor-trypsin complex per asymmetric unit, with a solvent content of 45%. © 2009 International Union of Crystallography. All rights reserved.
Citation: Acta Crystallographica Section F: Structural Biology and Crystallization Communications(2009), 65(11): 1179-1181
URI: https://doi.org/10.1107/S1744309109041694
http://repository.iitr.ac.in/handle/123456789/1706
Issue Date: 2009
Keywords: Kunitz-type inhibitors
Porcine pancreatic trypsin
Tamarind trypsin inhibitor
ISSN: 17443091
Author Scopus IDs: 35491886800
26431600400
57213408148
35491918300
35103173800
26431275200
57214355701
57203506001
55064809000
Author Affiliations: Tomar, S., Department of Biotechnology, Indian Institute of Technology, Roorkee, Roorkee 247667, India
Patil, D.N., Department of Biotechnology, Indian Institute of Technology, Roorkee, Roorkee 247667, India
Datta, M., Department of Biotechnology, Indian Institute of Technology, Roorkee, Roorkee 247667, India
Tapas, S., Department of Biotechnology, Indian Institute of Technology, Roorkee, Roorkee 247667, India
Preeti, Department of Biotechnology, Indian Institute of Technology, Roorkee, Roorkee 247667, India
Chaudhary, A., Department of Biotechnology, Indian Institute of Technology, Roorkee, Roorkee 247667, India
Sharma, A.K., Department of Biotechnology, Indian Institute of Technology, Roorkee, Roorkee 247667, India
Tomar, S., Department of Biotechnology, Indian Institute of Technology, Roorkee, Roorkee 247667, India
Kumar, P., Department of Biotechnology, Indian Institute of Technology, Roorkee, Roorkee 247667, India
Corresponding Author: Tomar, S.; Department of Biotechnology, Indian Institute of Technology, Roorkee, Roorkee 247667, India
Appears in Collections:Journal Publications [BT]

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