http://repository.iitr.ac.in/handle/123456789/1700| Title: | Isolation, purification, crystallization and preliminary crystallographic studies of chitinase from tamarind (Tamarindus indica) seeds |
| Authors: | Patil D.N. Datta M. Chaudhary A. Tomar, Shailly Kumar Sharma A. Kumar, Pravindra R.Manish |
| Published in: | Acta Crystallographica Section F: Structural Biology and Crystallization Communications |
| Abstract: | A protein with chitinase activity has been isolated and purified from tamarind (Tamarindus indica) seeds. N-terminal amino-acid sequence analysis of this protein confirmed it to be an 34 kDa endochitinase which belongs to the acidic class III chitinase family. The protein was crystallized by the vapour-diffusion method using PEG 4000. The crystals belonged to the tetragonal space group P41, with two molecules per asymmetric unit. Diffraction data were collected to a resolution of 2.6 Å. © 2009 International Union of Crystallography All rights reserved. |
| Citation: | Acta Crystallographica Section F: Structural Biology and Crystallization Communications(2009), 65(4): 343-345 |
| URI: | https://doi.org/10.1107/S1744309109006472 http://repository.iitr.ac.in/handle/123456789/1700 |
| Issue Date: | 2009 |
| Keywords: | Chitinases Tamarindus indicus |
| ISSN: | 17443091 |
| Author Scopus IDs: | 26431600400 57213408148 26431275200 57203506001 57190493325 55064809000 |
| Author Affiliations: | Patil, D.N., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, India Datta, M., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, India Chaudhary, A., Department of Biotechnology, I |
| Corresponding Author: | Kumar, P.; Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, India; email: kumarfbs@iitr.ernet.in |
| Appears in Collections: | Journal Publications [BT] |
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